SDS-Page
PHOSPHO1 recombinant protein
PHOSPHO1 Protein, Human, Recombinant (His Tag)
Purity
>92% as determined by SDS-PAGE
Synonyms
PHOSPHO1; PHOSPHO1 Protein; Human; Recombinant (His Tag); Human PHOSPHO1 Protein (His Tag); phosphatase; orphan 1; PHOSPHO1 recombinant protein
Host
E Coli
Purity/Purification
>92% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, 10% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Cys267
Species
Human
Predicted N Terminal
Met 1
Tag
N-His
Protein Construction
A DNA sequence encoding the human PHOSPHO1 isoform 1 (Q8TCT1-1)(Met 1-Cys 267) was expressed, with a polyhistide tag at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
References
Stewart AJ, et al. (2003) Comparative modelling of human PHOSPHO1 reveals a new group of phosphatases within the haloacid dehalogenase superfamily. Protein Eng. 16(12): 889-95.Camps M, et al. (2000) Dual specificity phosphatases: a gene family for control of MAP kinase function. FASEB J. 14(1): 6-16.Barford D. (1996) Molecular mechanisms of the protein serine/threonine phosphatases. Trends Biochem Sci. 21(11): 407-12.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,351 Da
NCBI Official Full Name
phosphoethanolamine/phosphocholine phosphatase isoform 1
NCBI Official Synonym Full Names
phosphatase, orphan 1
NCBI Official Symbol
PHOSPHO1
NCBI Protein Information
phosphoethanolamine/phosphocholine phosphatase
UniProt Protein Name
Phosphoethanolamine/phosphocholine phosphatase
Protein Family
UniProt Gene Name
PHOSPHO1
UniProt Entry Name
PHOP1_HUMAN
Uniprot Description
PHOSPHO1: Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization. Belongs to the HAD-like hydrolase superfamily. PHOSPHO family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Phosphatase; EC 3.1.3.75; Lipid Metabolism - glycerophospholipid
Chromosomal Location of Human Ortholog: 17q21.32
Cellular Component: cytosol
Molecular Function: pyrophosphatase activity; metal ion binding; phosphoric monoester hydrolase activity
Biological Process: dephosphorylation; phospholipid metabolic process; phosphatidylethanolamine biosynthetic process; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process; regulation of bone mineralization; endochondral ossification
Research Articles on PHOSPHO1
Similar Products
Product Notes
The PHOSPHO1 phospho1 (Catalog #AAA8121319) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Cys26 7. It is sometimes possible for the material contained within the vial of "PHOSPHO1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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