Mouse anti-Human RPN1 Monoclonal Antibody | anti-RPN1 antibody

RPN1 (Dolichyl-diphosphooligosaccharide--protein Glycosyltransferase Subunit 1, Dolichyl-diphosphooligosaccharide--protein Glycosyltransferase 67kD Subunit, Ribophorin I, RPN-I, Ribophorin-1, DKFZp686B16177) (Biotin)

Cat. Num. AAA6144133

• Gene Names: RPN1; OST1; RBPH1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPN1; Monoclonal Antibody; RPN1 (Dolichyl-diphosphooligosaccharide--protein Glycosyltransferase Subunit 1; Dolichyl-diphosphooligosaccharide--protein Glycosyltransferase 67kD Subunit; Ribophorin I; RPN-I; Ribophorin-1; DKFZp686B16177) (Biotin); anti-RPN1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2C10-2F6
• Specificity: Recognizes human RPN1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RPN1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-608 from human RPN1 (AAH10839) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: ASSEAPPLINEDVKRTVDLSSHLAKVTAEVVLAHLGGGSTSRATSFLLALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYSQDTFKVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSFKTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFPLFGGWKTHYIVGYNLPSYEYLYNLGDQYALKMRFVDHVFDEQVIDSLTVKIILPEGAKNIEIDSPYEISRAPDELHYTYLDTSGRPVIVAYKKNLVEQHIQDIVVHYTFNKVLMLQEPLLVVAAFYILFFTVIIYVRLDFSITKDPAAEARMKVACITEQVLTLVNKRIGLYRHFDETVNRYKQSRDISTLNSGKKSLEAEHKALTSEIALLQSRLKTEGSDLCDRVSEMQKLDAQVKELVLKSAVEAERLVAGKLKKDTYIENEKLISGKRQELVTKIDHILDAL
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged RPN1 is ~1ng/ml as a capture antibody.)

Related Product Information for anti-RPN1 antibody

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.

Product Categories/Family for anti-RPN1 antibody

Antibodies; Enzymes; Glycosyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 14789967
• NCBI GeneID: 6184
• Molecular Weight: 68,569 Da
• NCBI Official Full Name: Homo sapiens ribophorin I, mRNA
• NCBI Official Synonym Full Names: ribophorin I
• NCBI Official Symbol: RPN1
• NCBI Official Synonym Symbols: OST1; RBPH1
• NCBI Protein Information: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
• Protein Family: 26S proteasome regulatory

NCBI Description

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008]