Mouse RPA14 Monoclonal Antibody | anti-RPA14 antibody
RPA14 Monoclonal Antibody
Applications
Immunoblot, Western Blot, Immunoprecipitation
Synonyms
RPA14; Monoclonal Antibody; RPA14 Monoclonal Antibody; Anti-RPA14; anti-RPA14 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Specificity
Anti-RPA14 [Murine MAb recognizes Replication Protein A (14 kD), a single-stranded DNA binding protein essential in DNA replication, homologous recombination, and nucleotide excision repair.]
This antibody recognizes human and mouse RPA14. RPA (Replication Protein A) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32, and 14 kD. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination, and nucleotide excision repair.
This antibody recognizes human and mouse RPA14. RPA (Replication Protein A) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32, and 14 kD. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination, and nucleotide excision repair.
Form/Format
100 ug Protein G-purified antibody in PBS, pH 7.4
Sequence Length
137
Applicable Applications for anti-RPA14 antibody
Immunoblot, Immunoprecipitation
Application Notes
Immunoblotting, immunoprecipitation: use at 0.2-1 ug/ml.
Positive control: HeLa, Raji, and 3T3 cells and recombinant protein.
Positive control: HeLa, Raji, and 3T3 cells and recombinant protein.
Antigen
N-terminally 6x-His tagged fusion protein corresponding to fulllength human RPA14 expressed in E. coli.
Stabilizer
None
Preservatives
None. Available on request
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -70 degree C. Avoid multiple freeze thaw cycles.
Product Categories/Family for anti-RPA14 antibody
References
Iftode, C et al. 1999. Crit Rev Biochem Mol Biol 34: 141-180; Bochkarev, A et al. 1999. EMBO 18: 4498-4504; Dong, J et al. 1999. Biochem J 337: 311-317; Sibenaller, ZA et al. 1998. Biochemistry 37: 12496-12506; Stigger, E et al. 1998. J Biol Chem 273: 9337- 9343.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,585 Da
NCBI Official Full Name
Rpa14p
NCBI Official Symbol
RPA14
NCBI Protein Information
Rpa14p
UniProt Protein Name
DNA-directed RNA polymerase I subunit RPA14
Protein Family
UniProt Gene Name
RPA14
UniProt Entry Name
RPA14_YEAST
Uniprot Description
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. A14 seems to play a role in the stability of subunits ABC23 and A43. In vitro, the A14-A43 subcomplex binds single-stranded RNA. Ref.12
Subunit structure: Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits. Part of a Pol I subcomplex consisting of the subunits A14 and A43. Interacts with RPA43. Ref.9
Subcellular location: Nucleus › nucleolus Ref.10.
Post-translational modification: The N-terminus is blocked.
Miscellaneous: Present with 3100 molecules/cell in log phase SD medium. Ref.11
Similar Products
Product Notes
The RPA14 rpa14 (Catalog #AAA190409) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RPA14 can be used in a range of immunoassay formats including, but not limited to, Immunoblot, Immunoprecipitation. Immunoblotting, immunoprecipitation: use at 0.2-1 ug/ml. Positive control: HeLa, Raji, and 3T3 cells and recombinant protein. Researchers should empirically determine the suitability of the RPA14 rpa14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPA14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
