Mouse anti-Human RNH1 Monoclonal Antibody | anti-RNH1 antibody

RNH1 (Ribonuclease Inhibitor, Placental Ribonuclease Inhibitor, Placental RNase Inhibitor, Ribonuclease/Angiogenin Inhibitor 1, RAI, PRI, RNH) (HRP)

Cat. Num. AAA6154695

• Gene Names: RNH1; RAI; RNH
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RNH1; Monoclonal Antibody; RNH1 (Ribonuclease Inhibitor; Placental Ribonuclease Inhibitor; Placental RNase Inhibitor; Ribonuclease/Angiogenin Inhibitor 1; RAI; PRI; RNH) (HRP); anti-RNH1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 3F5
• Specificity: Recognizes human RNH1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 1697

• Applicable Applications for anti-RNH1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to human RNH1, aa1-462 (AAH11500) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MSLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDDCGLTEARCKDISSALRVNPALAELNLRSNELGDVGVHCVLQGLQTPSCKIQKLSLQNCCLTGAGCGVLSSTLRTLPTLQELHLSDNLLGDAGLQLLCEGLLDPQCRLEKLQLEYCSLSAASCEPLASVLRAKPDFKELTVSNNDINEAGVRVLCQGLKDSPCQLEALKLESCGVTSDNCRDLCGIVASKASLRELALGSNKLGDVGMAELCPGLLHPSSRLRTLWIWECGITAKGCGDLCRVLRAKESLKELSLAGNELGDEGARLLCETLLEPGCQLESLWVKSCSFTAACCSHFSSVLAQNRFLLELQISNNRLEDAGVRELCQGLGQPGSVLRVLWLADCDVSDSSCSSLAATLLANHSLRELDLSNNCLGDAGILQLVESVRQPGCLLEQLVLYDIYWSEEMEDRLQALEKDKPSLRVIS
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of RNH1 expression in transfected 293T cell line by RNH1 monoclonal antibody. ane 1: RNH1 transfected lysate (5kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-RNH1 antibody

Ribonuclease inhibitor 1 (RNH1) the placental ribonuclease inhibitor, is an acid protein with a molecular weight of 50kD. RNH1 is a member of a family of proteinaceous cytoplasmic RNase inhibitors that are expressed in many tissues and bind to both intracellular and extracellular RNases in the cytosol. RNH1 is a role in abolishing the ribonucleolytic and angiogenic activities of angiogenin.

Product Categories/Family for anti-RNH1 antibody

Antibodies; Enzymes; Ribonuclease

NCBI and Uniprot Product Information

• NCBI GI #: 15079320
• NCBI GeneID: 6050
• NCBI Official Full Name: Homo sapiens ribonuclease/angiogenin inhibitor 1, mRNA
• NCBI Official Synonym Full Names: ribonuclease/angiogenin inhibitor 1
• NCBI Official Symbol: RNH1
• NCBI Official Synonym Symbols: RAI; RNH
• NCBI Protein Information: ribonuclease inhibitor
• Protein Family: Ribonuclease inhibitor

NCBI Description

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]