Mouse RNF139 Monoclonal Antibody | anti-RNF139 antibody

RNF139 (RING Finger Protein 139, HRCA1, MGC31961, RCA1, TRC8) (PE)

Cat. Num. AAA6187182

• Gene Names: RNF139; RCA1; TRC8; HRCA1
• Applications: Western Blot
• Purity: Purified
• Synonyms: RNF139; Monoclonal Antibody; RNF139 (RING Finger Protein 139; HRCA1; MGC31961; RCA1; TRC8) (PE); Ring Finger Protein 139; TRC8; anti-RNF139 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2D5
• Specificity: Recognizes RNF139.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RNF139 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: RNF139 (NP_009149, 565aa-664aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged RNF139 is 0.03 ng/ml as a capture antibody.)

Product Categories/Family for anti-RNF139 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 21314654
• NCBI GeneID: 11236
• NCBI Accession #: NP_009149
• NCBI GenBank Nucleotide #: NM_007218
• UniProt Accession #: Q8WU17
• Molecular Weight: 76kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF139
• NCBI Official Synonym Full Names: ring finger protein 139
• NCBI Official Symbol: RNF139
• NCBI Official Synonym Symbols: RCA1; TRC8; HRCA1
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF139
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF139
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF139
• UniProt Entry Name: RN139_HUMAN

NCBI Description

The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP. [provided by RefSeq, Jul 2008]

Uniprot Description

RNF139: E3-ubiquitin ligase; acts as a negative regulator of the cell proliferation through mechanisms involving G2/M arrest and cell death. Required for MHC class I ubiquitination in cells expressing the cytomegalovirus protein US2 before dislocation from the endoplasmic reticulum (ER). Affects SREBP processing by hindering the SREBP/SCAP complex translocation from the ER to the Golgi, thereby reducing SREBF2 target gene expression. Required for INSIG1 ubiquitination. May be required for EIF3 complex ubiquitination. May function as a signaling receptor. Defects in RNF139 may be a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. A chromosomal aberration involving RNF139 has been found in a lymphoblastoid cell line established from a family with renal cell carcinoma and thyroid carcinoma. Translocation (3;8)(q14.2;q24.1) with FHIT. RNF139 is found to be fused to FHIT and disrupted within the sterol-sensing domain. In contrast, the FHIT coding region is maintained and expressed. Sporadic cases of renal carcinoma, where an acquired mutation in RNF139 results in the duplication of 12 nucleotides in the 5'-UTR, has also been identified.

Protein type: Ligase; Membrane protein, multi-pass; EC 6.3.2.-; EC 6.3.2.19; Membrane protein, integral; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; protease binding; small conjugating protein ligase activity; zinc ion binding; ubiquitin-protein ligase activity; receptor activity; ligase activity

Biological Process: negative regulation of cell proliferation; protein destabilization; regulation of protein ubiquitination; negative regulation of translation; protein ubiquitination

Disease: Renal Cell Carcinoma, Nonpapillary