Rabbit anti-Mouse, Human VGLU2 Polyclonal Antibody | anti-VGLU2 antibody

VGLU2, CT (Slc17a6, Dnpi, Vglut2, Vesicular glutamate transporter 2, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6) (APC)

Cat. Num. AAA6362566

• Gene Names: Slc17a6; DNPI; VGLUT2; 2900073D12Rik
• Reactivity: Mouse, Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: VGLU2; Polyclonal Antibody; CT (Slc17a6; Dnpi; Vglut2; Vesicular glutamate transporter 2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6) (APC); Slc17a6; Solute carrier family 17 member 6; anti-VGLU2 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse VGLU2. Species Crossreactivity: human
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-VGLU2 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 507-535 in the C-terminal region of mouse VGLU2, Swiss/UniProt Accession: Q8BLE7. Species sequence homology: bovine, rat
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse cerebellum, brain tissue lysates (35ug/lane) usingMBS6004735. VGLU2 (arrow) was detected usingMBS6004735.)

Testing Data

(All lanes: MBS6004735 (1:1000-1:2000) Lane 1: Human brain lysate Lane 2: Human fetal brain lysate Lane 3: Human cerebellum lysate Lane 4: Mouse cerebellum lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 65kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

(All lanes: MBS6004735 (1:2000) Lane 1: Human fetal brain lysate Lane 2: Human cerebellum lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 65kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain reacted withMBS6004735, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6004735 for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-VGLU2 antibody

Antibodies; Transport Proteins

References

1. Birgner, C., et.al., Proc. Natl. Acad. Sci. U.S.A. 107 (1), 389-394 (2010). 2. Renier, N., et.al., PLoS Biol. 8 (3), E1000325 (2010). 3. Rose, M.F., et.al., Proc. Natl. Acad. Sci. U.S.A. 106 (52), 22462-22467 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 188219544
• NCBI GeneID: 140919
• NCBI Accession #: NP_543129.3
• NCBI GenBank Nucleotide #: NM_080853.3
• UniProt Accession #: Q8BLE7; Q920B7
• Molecular Weight: 64,561 Da
• NCBI Official Full Name: vesicular glutamate transporter 2
• NCBI Official Synonym Full Names: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
• NCBI Official Symbol: Slc17a6
• NCBI Official Synonym Symbols: DNPI; VGLUT2; 2900073D12Rik
• NCBI Protein Information: vesicular glutamate transporter 2; differentiation-associated BNPI; solute carrier family 17 member 6; solute carrier family 17 (vesicular glutamate transporter), member 6; differentiation-associated Na(+)-dependent inorganic phosphate cotransporter
• UniProt Protein Name: Vesicular glutamate transporter 2
• UniProt Gene Name: Slc17a6
• UniProt Synonym Gene Names: Dnpi; Vglut2; VGluT2
• UniProt Entry Name: VGLU2_MOUSE

Uniprot Description

SLC17A6: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral

Cellular Component: synaptic vesicle; neuron projection; membrane; early endosome; integral to membrane; synapse; cytoplasmic vesicle; cell junction

Molecular Function: symporter activity; L-glutamate transmembrane transporter activity

Biological Process: neurotransmitter transport; transport; sodium ion transport; neurotransmitter uptake; ion transport; transmembrane transport