Mouse anti-Human Ribonuclease A Monoclonal Antibody | anti-RNASE1 antibody

Anti-Ribonuclease A Antibody, Mouse Monoclonal

Cat. Num. AAA8106945

• Gene Names: RNASE1; RAC1; RIB1; RNS1
• Reactivity: Human
• Applications: ELISA, ELISA
• Purity: Protein A
• Synonyms: Ribonuclease A; Monoclonal Antibody; Anti-Ribonuclease A Antibody; Mouse Monoclonal; RNase A/Ribonuclease A/RNASE1 Antibody; Mouse MAb; ribonuclease; RNase A family; 1 (pancreatic); Anti-RAC1 Antibody; Anti-RIB1 Antibody; Anti-RNS1 Antibody; anti-RNASE1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 02
• Specificity: Human RNase A/Ribonuclease A/RNASE1.; No cross-reactivity in ELISA with Human cell lysate (293 cell line)
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-RNASE1 antibody: ELISA (EIA), ELISA (EIA) (Cap)
• Application Notes: ELISA: 1:1000-1:2000; ELISA (Cap): 1:250-1:2000; ELISA (Cap): This antibody will detect Human RNase A/Ribonuclease A/RNASE1 in ELISA pair set.; In a sandwich ELISA, it can be used as capture antibody.
• Immunogen: Recombinant Human RNase A/Ribonuclease A/RNASE1 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RNase A/Ribonuclease A/RNASE1 (rh RNase A/Ribonuclease A/RNASE1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-RNASE1 antibody

RNase A, also known as ribonuclease A and RNASE1, belongs to ribonuclease A superfamily. It is a pancreatic-type of secretory ribonuclease. RNase A is a basic protein and its many positive charges are consistent with its binding to RNA (a poly-anion). More generally, RNase A is unusually polar or, rather, unusually lacking in hydrophobic groups, especially aliphatic ones. As an endonuclease, RNase A cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2', 3'-cyclic nucleotides, with a pH optimum near 8.0. RNase A is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

References

Tubert P, et al. (2011) Interactions crucial for three-dimensional domain swapping in the HP-RNase variant PM8. Biophys J. 101(2):459-67.Vinayagam A, et al. (2011) A directed protein interaction network for investigating intracellular signal transduction. Sci Signal. 4(189):rs8.Fischer S, et al. (2011) Expression and localisation of vascular ribonucleases in endothelial cells. Thromb Haemost. 105(2):345-55.

NCBI and Uniprot Product Information

• NCBI GI #: 4506547
• NCBI GeneID: 6035
• NCBI Accession #: NP_002924.1
• NCBI GenBank Nucleotide #: NM_002933.5
• UniProt Accession #: P07998; Q16830; Q16869; Q1KHR2; Q6ICS5; Q9UCB4; Q9UCB5; B2R589; D3DS06
• Molecular Weight: 17,644 Da
• NCBI Official Full Name: ribonuclease pancreatic
• NCBI Official Synonym Full Names: ribonuclease A family member 1, pancreatic
• NCBI Official Symbol: RNASE1
• NCBI Official Synonym Symbols: RAC1; RIB1; RNS1
• NCBI Protein Information: ribonuclease pancreatic
• UniProt Protein Name: Ribonuclease pancreatic
• Protein Family: Ribonuclease
• UniProt Gene Name: RNASE1
• UniProt Synonym Gene Names: RIB1; RNS1; RNase 1; RNase A

NCBI Description

This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA.