Mouse RHODOPSIN Monoclonal Antibody | anti-RHO antibody

MOUSE ANTI RHODOPSIN

Cat. Num. AAA219700

• Reactivity: Amphibia, Mammals
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Synonyms: RHODOPSIN; Monoclonal Antibody; MOUSE ANTI RHODOPSIN; anti-RHO antibody

• Host: Mouse
• Reactivity: Amphibia, Mammals
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1D4
• Specificity: This product recognises the sequence TETSQVAPA corresponding to aa 340 to 348 of bovine rhodopsin, a pigment of the retina responsible for the formation of photoreceptor cells and the first events in the perception of light. Rhodopsin consists of the protein opsin and a reversibly bound cofactor, retinal, a photoreactive chromophore. Upon light absorption the 11-cis-retinal is isomerised to all-trans retinal, leading to a change in the shape of rhodopsin. The change activates the associated G protein and triggers a second messenger cascade, eventually resulting in the transmission of a nerve impulse to the brain. The trans-retinal is released and converted back into 11-cis-retinal for re-entry into the cycle. ; ; Defects in the gene cause various retinopathies such as retinitis pigmentosa and X-linked congenital stationary night blindness.
• Form/Format: Purified; Purified IgG - liquid
• Sequence Length: 348

• Applicable Applications for anti-RHO antibody: Immunoprecipitation (IP), Immunohistology Paraffin, Western Blot (WB)
• Application Notes: Immunohistology - Paraffin: Maximum Dilution: 1/100;; Western Blotting: Maximum Dilution: 1/1000
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3); 0.01% Bovine Serum Albumin; 50% Glycerol ; Preparation
• Immunogen: Bovine rhodopsin; Histology Positive Control Tissue
• Target Species: Bovine
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-RHO antibody

This product recognises the sequence TETSQVAPA corresponding to aa 340 to 348 of bovine rhodopsin, a pigment of the retina responsible for the formation of photoreceptor cells and the first events in the perception of light. Rhodopsin consists of the protein opsin and a reversibly bound cofactor, retinal, a photoreactive chromophore. Upon light absorption the 11-cis-retinal is isomerised to all-trans retinal, leading to a change in the shape of rhodopsin. The change activates the associated G protein and triggers a second messenger cascade, eventually resulting in the transmission of a nerve impulse to the brain. The trans-retinal is released and converted back into 11-cis-retinal for re-entry into the cycle. Defects in the gene cause various retinopathies such as retinitis pigmentosa and X-linked congenital stationary night blindness.

NCBI and Uniprot Product Information

• NCBI GI #: 62460472
• NCBI GeneID: 509933
• NCBI Accession #: NP_001014890.1
• NCBI GenBank Nucleotide #: NM_001014890.1
• UniProt Accession #: P02699
• Molecular Weight: 39,008 Da
• NCBI Official Full Name: rhodopsin
• NCBI Official Synonym Full Names: rhodopsin
• NCBI Official Symbol: RHO
• NCBI Protein Information: rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant)
• UniProt Protein Name: Rhodopsin
• Protein Family: Rhodopsin
• UniProt Gene Name: RHO
• UniProt Entry Name: OPSD_BOVIN

Uniprot Description

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal ().