Mouse RhoA,B,C Monoclonal Antibody | anti-ARHGAP5 antibody

RhoA,B,C (GDP/GTP Binding Protein)

Cat. Num. AAA606927

• Gene Names: ARHGAP5; GFI2; p190-B; RhoGAP5; p190BRhoGAP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: RhoA; B; C; Monoclonal Antibody; C (GDP/GTP Binding Protein); Anti -RhoA; anti-ARHGAP5 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4F5
• Specificity: Recognizes human Rho (-A, -B, -C), Mr 22kD. Species Crossreactivity: rat and mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide, before the addition of glycerol to 30%.

• Applicable Applications for anti-ARHGAP5 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Immunoblot, Immunocytochemistry and Immunofluorescence.; Dilution: Immunoblot: 0.5-3ug/ml detects Rho in RIPA lysates from Jurkat and HL-60 cells. HL-60 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with 3ug/ml R1998-01. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.; Immunocytochemistry: Reported to detect Rho in cells via immunofluorescence.
• Immunogen: Recombinant human RhoA containing amino acids 1-155.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunoblot (IB)

(IB: HL-60 cell lysate was probed with anti-Rho (-A, -B, -C) (3ug/ml).)

Product Categories/Family for anti-ARHGAP5 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 71834861
• NCBI GeneID: 394
• NCBI Accession #: NP_001164.2
• NCBI GenBank Nucleotide #: NM_001173.2
• UniProt Accession #: Q13017; Q05BE8; Q05BU8; Q59ER0; Q6DHZ3; A1L375; A1L376; A8KAA1; D3DS89; D3DS90
• Molecular Weight: 172,460 Da
• NCBI Official Full Name: rho GTPase-activating protein 5 isoform b
• NCBI Official Synonym Full Names: Rho GTPase activating protein 5
• NCBI Official Symbol: ARHGAP5
• NCBI Official Synonym Symbols: GFI2; p190-B; RhoGAP5; p190BRhoGAP
• NCBI Protein Information: rho GTPase-activating protein 5; p105 RhoGAP; growth factor independent 2; p100 RasGAP-associated p105 protein; rho-type GTPase-activating protein 5
• UniProt Protein Name: Rho GTPase-activating protein 5
• UniProt Gene Name: ARHGAP5
• UniProt Synonym Gene Names: RHOGAP5
• UniProt Entry Name: RHG05_HUMAN

NCBI Description

Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ARHGAP5: a GTPase activating protein for Rho family members. May play a role in the reduction of the p21rasGTPase-activating potential of p120GAP. Negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP.

Protein type: Motility/polarity/chemotaxis; GAPs, Rac/Rho; GAPs

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; SH2 domain binding

Biological Process: regulation of small GTPase mediated signal transduction; mammary gland development; metabolic process; small GTPase mediated signal transduction; positive regulation of mesenchymal cell proliferation; cell adhesion; regulation of cell size; Rho protein signal transduction; positive regulation of cell migration