Mouse anti-Human RFC5 Monoclonal Antibody | anti-RFC5 antibody

RFC5 (Replication Factor C Subunit 5, Activator 1 Subunit 5, Replication Factor C 36kD Subunit, RF-C 36kD Subunit, RFC36, Activator 1 36kD Subunit, A1 36kD Subunit, MGC1155) (HRP)

Cat. Num. AAA6154605

• Gene Names: RFC5; RFC36
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RFC5; Monoclonal Antibody; RFC5 (Replication Factor C Subunit 5; Activator 1 Subunit 5; Replication Factor C 36kD Subunit; RF-C 36kD Subunit; RFC36; Activator 1 36kD Subunit; A1 36kD Subunit; MGC1155) (HRP); anti-RFC5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1B1
• Specificity: Recognizes human RFC5.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 1953

• Applicable Applications for anti-RFC5 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to human RFC5, aa1-341 (AAH13961) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: METSALKQQEQPAATKIRNLPWVEKYRPQTLNDLISHQDILSTIQKFINEDRLPHLLLYGPPGTGKTSTILACAKQLYKDKEFGSMVLELNASDDRGIDIIRGPILSFASTRTIFKKGFKLVILDEADAMTQDAQNALRRVIEKFTENTRFCLICNYLSKIIPALQSRCTRFRFGPLTPELMAPRLEHVVEEEKVDISEDGMKALVTLSSGDMRRALNILQSTNMAFGKVTEETVYTCTGHPLKSDIANILDWMLNQDFTTAYRNITELKTLKGLALHDILTEIHLFVHRVDFPSSVRIHLLTKMADIEYRLSVGTNEKIQLSSLIAAFQVTRDLIVAEA
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RFC5 monoclonal antibody. Western Blot analysis of RFC5 expression in Jurkat.)

Related Product Information for anti-RFC5 antibody

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36kD. RFC5 is the 36kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40kD subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.

Product Categories/Family for anti-RFC5 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 33990991
• NCBI GeneID: 5985
• NCBI Official Full Name: Homo sapiens replication factor C (activator 1) 5, 36.5kDa, mRNA
• NCBI Official Synonym Full Names: replication factor C subunit 5
• NCBI Official Symbol: RFC5
• NCBI Official Synonym Symbols: RFC36
• NCBI Protein Information: replication factor C subunit 5
• Protein Family: Replication factor

NCBI Description

This gene encodes the smallest subunit of the replication factor C complex, which consists of five distinct subunits (140, 40, 38, 37, and 36 kDa) and is required for DNA replication. This subunit interacts with the C-terminal region of proliferating cell nuclear antigen and is required to open and load proliferating cell nuclear antigen onto DNA during S phase. It is a member of the AAA+ (ATPases associated with various cellular activities) ATPase family and forms a core complex with the 38 and 40 kDa subunits that possesses DNA-dependent ATPase activity. A related pseudogene has been identified on chromosome 9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]