Rabbit anti-Human RFC5 Polyclonal Antibody | anti-RFC5 antibody

RFC5, ID (R185) (Replication Factor C Subunit 5, Activator 1 Subunit 5, Replication Factor C 36kD Subunit, RF-C 36kD Subunit, RFC36, Activator 1 36kD Subunit, A1 36kD Subunit, RFC5)

Cat. Num. AAA627350

• Gene Names: RFC5; RFC36; MGC1155
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: RFC5; Polyclonal Antibody; ID (R185) (Replication Factor C Subunit 5; Activator 1 Subunit 5; Replication Factor C 36kD Subunit; RF-C 36kD Subunit; RFC36; Activator 1 36kD Subunit; A1 36kD Subunit; RFC5); Anti -RFC5; anti-RFC5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RFC5.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-RFC5 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the Center region of human RFC5 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-RFC5 Antibody (Center R185) in Hela cell line lysates (35ug/lane). RFC5(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-RFC5 Antibody (Center R185) in rat PASMC cell line lysates (35ug/lane). RFC5(arrow) was detected using the purified Pab( Provided by Hirotaka Ata).)

Western Blot (WB)

(Western blot analysis of RFC5 (arrow) using rabbit polyclonal RFC5 Antibody (Center R185) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFC5 gene.)

Flow Cytometry (FC/FACS)

(RFC5 Antibody (Center R185) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RFC5 antibody

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36kD. RFC5 is the 36kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40kD subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.

Product Categories/Family for anti-RFC5 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 194306569
• NCBI GeneID: 5985
• NCBI Accession #: NP_001123584.1
• NCBI GenBank Nucleotide #: NM_001130112.2
• UniProt Accession #: P40937; Q59GW7; Q6LES9
• Molecular Weight: 38,497 Da
• NCBI Official Full Name: replication factor C subunit 5 isoform 3
• NCBI Official Synonym Full Names: replication factor C (activator 1) 5, 36.5kDa
• NCBI Official Symbol: RFC5
• NCBI Official Synonym Symbols: RFC36; MGC1155
• NCBI Protein Information: replication factor C subunit 5; A1 36 kDa subunit; OTTHUMP00000209892; OTTHUMP00000209893; RF-C 36 kDa subunit; RFC, 36.5 kD subunit; replication factor C, 36-kDa subunit
• UniProt Protein Name: Replication factor C subunit 5
• Protein Family: Replication factor
• UniProt Gene Name: RFC5
• UniProt Entry Name: RFC5_HUMAN

NCBI Description

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9. [provided by RefSeq]

Uniprot Description

RFC5: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. Belongs to the activator 1 small subunits family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 12q24.23

Cellular Component: nucleoplasm; DNA replication factor C complex; nucleus

Molecular Function: protein binding; enzyme binding; ATP binding; DNA clamp loader activity

Biological Process: telomere maintenance via semi-conservative replication; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA strand elongation during DNA replication; DNA repair; DNA-dependent DNA replication; DNA replication; telomere maintenance