Mouse Reticulon-1C / NSP-C Monoclonal Antibody | anti-RTN1 antibody

Mouse anti Reticulon-1C / NSP-C

Cat. Num. AAA570062

• Gene Names: RTN1; NSP
• Reactivity: Human, Rat, Swine
• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
• Synonyms: Reticulon-1C / NSP-C; Monoclonal Antibody; Mouse anti Reticulon-1C / NSP-C; anti-RTN1 antibody

• Host: Mouse
• Reactivity: Human, Rat, Swine
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: RNL-4
• Specificity: RNL-4 recognizes an epitope loCated within the first 20 amino acids of Reticulon-1C (NSP-C). RNL-4 reacts with peripheral nerves and ganglia of various tissues and cross-reacts with smooth muscle cells and myoepithelium. In the central nervous system it reacts with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative. Reticulon-1 has been found to indiCate neuronal differentiation and to be downregulated in neurological pathologies.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-RTN1 antibody: Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Western Blot (WB)
• Application Notes: RNL-4 is useful for immunocytochemistry, immunohistochemistry on frozen tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:50 - 1:100 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting appliCations.
• Source Note: RNL-4 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells, with spleen cells from a BALB/c Mouse immunized with a synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Reticulon-1C.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

Testing Data

(Figure 1: Immunoblotting of RNL-4 recognizing NSP-C Reticulon 1-C (23 kDa) in extract from neuroblastoma cell line LA-N-5 )

Related Product Information for anti-RTN1 antibody

Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to Human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer identification Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).

Product Categories/Family for anti-RTN1 antibody

Membrane; Cancer; Neurobiology

References

Senden, N. H., Timmer, E. D., Boers, J. E., van de Velde, H. J., Roebroek, A. J., Van de Ven, W. J., Broers, J. L., and Ramaekers, F. C. (1996). Neuroendocrine-specific protein C (NSP-C): subcellular localization and differential expression in relation to NSP-A, Eur J Cell Biol 69, 197-213. Senden, N. H., Timmer, E. D., de Bruine, A., Wagenaar, S. S., Van de Velde, H. J., Roebroek, A. J., Van de Ven, W. J., Broers, J. L., and Ramaekers, F. C. (1997). A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers, J Pathol 182, 13-21. Hens, J., Nuydens, R., Geerts, H., Senden, N. H., Van de Ven, W. J., Roebroek, A. J., van de Velde, H. J., Ramaekers, F. C., and Broers, J. L. (1998). Neuronal differentiation is accompanied by NSP-C expression, Cell Tissue Res 292, 229-37. Kim, S. H., Yoo, B. C., Broers, J. L., Cairns, N., and Lubec, G. (2000). Neuroendocrine-specific protein C, a marker of neuronal differentiation, is reduced in brain of patients with Down syndrome and Alzheimer's disease, Biochem Biophys Res Commun 276, 329-34.

NCBI and Uniprot Product Information

• NCBI GI #: 45827776
• NCBI GeneID: 6252
• NCBI Accession #: NP_996734.1
• NCBI GenBank Nucleotide #: NM_206852.2
• UniProt Accession #: Q16799; Q16800; Q16801; Q5BKZ4; Q9BQ59
• Molecular Weight: 83,618 Da
• NCBI Official Full Name: reticulon-1 isoform C
• NCBI Official Synonym Full Names: reticulon 1
• NCBI Official Symbol: RTN1
• NCBI Official Synonym Symbols: NSP
• NCBI Protein Information: reticulon-1; neuroendocrine-specific protein
• UniProt Protein Name: Reticulon-1
• Protein Family: Reticulon
• UniProt Gene Name: RTN1
• UniProt Synonym Gene Names: NSP
• UniProt Entry Name: RTN1_HUMAN

NCBI Description

This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. This gene is considered to be a specific marker for neurological diseases and cancer, and is a potential molecular target for therapy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2011]

Uniprot Description

RTN1: May be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell development/differentiation; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane

Molecular Function: protein binding

Biological Process: neuron differentiation