Mouse Reticulon-1A/B / NSP-A/B Monoclonal Antibody

Mouse anti Reticulon-1A/B / NSP-A/B

Cat. Num. AAA570124

• Reactivity: Human, Monkey, Rabbit
• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
• Synonyms: Reticulon-1A/B / NSP-A/B; Monoclonal Antibody; Mouse anti Reticulon-1A/B / NSP-A/B; anti-Reticulon-1A/B / NSP-A/B antibody

• Host: Mouse
• Reactivity: Human, Monkey, Rabbit
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: RNL-2
• Specificity: RNL-2 recognizes an epitope loCated within the region of amino acids 421-589 of the neuro-endocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, RNL-2 reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-Reticulon-1A/B / NSP-A/B antibody: Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
• Application Notes: RNL-2 is useful for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
• Source Note: RNL-2 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with the small cell lung cancer cell line NCI-H82.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

Related Product Information for anti-Reticulon-1A/B / NSP-A/B antibody

Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to Human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer identification Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).

Product Categories/Family for anti-Reticulon-1A/B / NSP-A/B antibody

Membrane; Cancer; Neurobiology

References

Broers, J. L., Mijnheere, E. P., Rot, M. K., Schaart, G., Sijlmans, A., Boerman, O. C., and Ramaekers, F. C. (1991). Novel antigens characteristic of neuroendocrine malignancies, Cancer 67, 619-33. Roebroek, A. J., van de Velde, H. J., Van Bokhoven, A., Broers, J. L., Ramaekers, F. C., and Van de Ven, W. J. (1993). Cloning and expression of alternative transcripts of a novel neuroendocrine-specific gene and identifiCation of its 135-kDa translational product, J Biol Chem 268, 13439-47. Senden, N. H., van de Velde, H. J., Broers, J. L., Timmer, E. D., Roebroek, A. J., van de Ven, W. J., and Ramaekers, F. C. (1994). Cluster-10 lung-cancer antibodies recognize NSPs, novel neuro-endocrine proteins associated with membranes of the endoplasmic reticulum, Int J Cancer Suppl 8, 84-8. Senden, N. H., Timmer, E. D., Boers, J. E., van de Velde, H. J., Roebroek, A. J., Van de Ven, W. J., Broers, J. L., and Ramaekers, F. C. (1996). Neuroendocrine-specific protein C (NSP-C): subcellular localization and differential expression in relation to NSP-A, Eur J Cell Biol 69, 197-213. Senden, N. H., Timmer, E. D., de Bruine, A., Wagenaar, S. S., Van de Velde, H. J., Roebroek, A. J., Van de Ven, W. J., Broers, J. L., and Ramaekers, F. C. (1997). A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers, J Pathol 182, 13-21.