Mouse anti-Human RBM3 Monoclonal Antibody | anti-RBM3 antibody

Mouse Monoclonal [clone 4D6] (IgG1,k) to Human RBM3

Cat. Num. AAA245695

• Gene Names: RBM3; RNPL; IS1-RNPL
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: RBM3; Monoclonal Antibody; Mouse Monoclonal [clone 4D6] (IgG1; k) to Human RBM3; Anti-RBM3 Antibody (clone 4D6) IHC-plus; IS1-RNPL; RNA binding motif protein 3; RNPL; RNA-binding motif protein 3; Putative RNA-binding protein 3; Human RBM3; anti-RBM3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4D6
• Specificity: Human RBM3
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 157

• Applicable Applications for anti-RBM3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (10 - 20 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
• Target Species: Human
• Immunogen Description: RBM3 (NP_006734, 1 a.a. ~ 81 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: RBM3 antibody was raised against rBM3 (NP_006734, 1 a.a. ~ 81 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Aliquot and store at -20 degree C or -80 degree C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-RBM3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

ELISA (EIA)

(Detection limit for recombinant GST tagged RBM3 is 0.1 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 5803137
• NCBI GeneID: 5935
• NCBI Accession #: NP_006734.1
• NCBI GenBank Nucleotide #: NM_006743.4
• UniProt Accession #: P98179
• Molecular Weight: 17,170 Da
• NCBI Official Full Name: putative RNA-binding protein 3
• NCBI Official Synonym Full Names: RNA binding motif (RNP1, RRM) protein 3
• NCBI Official Symbol: RBM3
• NCBI Official Synonym Symbols: RNPL; IS1-RNPL
• NCBI Protein Information: putative RNA-binding protein 3; RNA-binding motif protein 3
• UniProt Protein Name: Putative RNA-binding protein 3
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBM3
• UniProt Synonym Gene Names: RNPL
• UniProt Entry Name: RBM3_HUMAN

NCBI Description

This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2008]

Uniprot Description

RBM3: Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation.

Protein type: RNA-binding; Translation; Ribosomal; RNA processing

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: cytoplasm; dendrite; nucleus; large ribosomal subunit

Molecular Function: protein binding; RNA binding; nucleotide binding; ribosomal large subunit binding

Biological Process: regulation of translation; RNA processing; miRNA-mediated gene silencing, production of miRNAs; positive regulation of translation; translation; response to cold