Mouse RBBP7 Monoclonal Antibody | anti-RBBP7 antibody

RBBP7 antibody

Cat. Num. AAA833109

• Gene Names: RBBP7; RbAp46
• Applications: Immunohistochemistry, Western Blot
• Purity: RBBP7 antibody was purified by affinity chromatography.
• Synonyms: RBBP7; Monoclonal Antibody; RBBP7 antibody; Monoclonal RBBP7; Anti-RBBP7; Retinoblastoma Binding Protein 7; MGC138867; MGC138868; RbAp46; RBBP 7; RBBP-7; anti-RBBP7 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5A4
• Purity/Purification: RBBP7 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 425

• Applicable Applications for anti-RBBP7 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:150; WB: 1:500
• Biological Significance: This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation.
• Cross-Reactivity: Human
• Immunogen: RBBP7 antibody was raised in mouse using a full length human recombinnat protein of RBBP7 (NP_002884) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RBBP7 protein (Right) or empty vector (Left) detected with RBBP7 antibody)

Related Product Information for anti-RBBP7 antibody

Mouse monoclonal RBBP7 antibody

Product Categories/Family for anti-RBBP7 antibody

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4506439
• NCBI GeneID: 5931
• NCBI Accession #: NP_002884
• NCBI GenBank Nucleotide #: NM_002893.3
• UniProt Accession #: Q16576; Q5JP00
• Molecular Weight: 52,314 Da
• NCBI Official Full Name: histone-binding protein RBBP7 isoform 2
• NCBI Official Synonym Full Names: retinoblastoma binding protein 7
• NCBI Official Symbol: RBBP7
• NCBI Official Synonym Symbols: RbAp46
• NCBI Protein Information: histone-binding protein RBBP7
• UniProt Protein Name: Histone-binding protein RBBP7
• Protein Family: Histone-binding protein
• UniProt Gene Name: RBBP7
• UniProt Synonym Gene Names: RBAP46; RBBP-7
• UniProt Entry Name: RBBP7_HUMAN

NCBI Description

This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

RBBP7: Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. Binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin. Subunit of the type B histone acetyltransferase (HAT) complex, composed of RBBP7 and HAT1. Subunit of the core histone deacetylase (HDAC) complex, which is composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core HDAC complex associates with SIN3A, ARID4B/SAP180, SAP18, SAP30, SAP130, SDS3/SAP45 and possibly ARID4A/RBP1 and ING1 to form the SIN3 HDAC complex. The core HDAC complex may also associate with MTA2, MBD3, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylase complex (the NuRD complex). The NuRD complex may also interact with MBD3L1 and MBD3L2. Interacts with MTA1. Subunit of the PRC2/EED-EZH2 complex, which is composed of at least EED, EZH2, RBBP4, RBBP7 and SUZ12. The PRC2/EED-EZH2 complex may also associate with HDAC1. Part of the nucleosome remodeling factor (NURF) complex which consists of SMARCA1; BPTF; RBBP4 and RBBP7. Interacts with the viral protein- binding domain of the retinoblastoma protein (RB1). Interacts with CREBBP, and this interaction may be enhanced by the binding of phosphorylated CREB1 to CREBBP. Interacts with BRCA1, HDAC7 and SUV39H1. Belongs to the WD repeat RBAP46/RBAP48/MSI1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: nucleoplasm; ESC/E(Z) complex; NuRD complex; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: nucleosome assembly; cell proliferation; establishment and/or maintenance of chromatin architecture; DNA replication-independent nucleosome assembly at centromere; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; multicellular organismal development; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; DNA replication; regulation of gene expression, epigenetic