Mouse anti-Human, Mouse RAPGEFL1 Monoclonal Antibody | anti-RAPGEFL1 antibody

RAPGEFL1 (Rap guanine nucleotide exchange factor-like protein 1, Link guanine nucleotide exchange factor II)

Cat. Num. AAA606014

• Gene Names: RAPGEFL1; MGC134798; MGC134799; Link-GEFII
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: RAPGEFL1; Monoclonal Antibody; RAPGEFL1 (Rap guanine nucleotide exchange factor-like protein 1; Link guanine nucleotide exchange factor II); Anti -RAPGEFL1 (Rap guanine nucleotide exchange factor-like protein 1; anti-RAPGEFL1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9G430
• Specificity: Recognizes human RAPGELF1. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.

• Applicable Applications for anti-RAPGEFL1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.
• Immunogen: Partial recombinant human RAPGELF1 (1-100) with a GST tag.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degree C. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot detection of RAPGEFL1 expression in human neuroblastoma cell lysate.)

ELISA (EIA)

(Sandwich ELISA using the anti-RAPGEFL1 monoclonal antibody as capture antibody. The detection limit for GST-tagged recombinant RAPGEF1 is 1 ng/ml.)

Related Product Information for anti-RAPGEFL1 antibody

RAPGEFL1 is a 456 aa protein with an estimated molecular weight of ~52kD. It is a guanine-nucleotide releasing factor and contains one Ras-GEF domain.

Product Categories/Family for anti-RAPGEFL1 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 7705939
• NCBI GeneID: 51195
• NCBI Accession #: NP_057423.1
• NCBI GenBank Nucleotide #: NM_016339.3
• UniProt Accession #: Q9UHV5
• Molecular Weight: 73,265 Da
• NCBI Official Full Name: rap guanine nucleotide exchange factor-like 1
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor (GEF)-like 1
• NCBI Official Symbol: RAPGEFL1
• NCBI Official Synonym Symbols: MGC134798; MGC134799; Link-GEFII
• NCBI Protein Information: rap guanine nucleotide exchange factor-like 1; link GEFII; OTTHUMP00000237586; OTTHUMP00000237588; OTTHUMP00000237589; Link guanine nucleotide exchange factor II; RAP guanine-nucleotide-exchange factor (GEF)-like 1
• UniProt Protein Name: Rap guanine nucleotide exchange factor-like 1
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: RAPGEFL1
• UniProt Entry Name: RPGFL_HUMAN

Uniprot Description

RAPGEFL1: Probable guanine nucleotide exchange factor (GEF). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, Ras; GAPs

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: membrane

Molecular Function: guanyl-nucleotide exchange factor activity

Biological Process: G-protein coupled receptor protein signaling pathway; nervous system development; small GTPase mediated signal transduction; positive regulation of GTPase activity