Western Blot (WB)
(Western Blot analysis of RAPGEF1 expression in transfected 293T cell line by RAPGEF1 polyclonal antibody. Lane 1: RAPGEF1 transfected lysate (120.45kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human RAPGEF1 Polyclonal Antibody | anti-RAPGEF1 antibody
RAPGEF1 (Rap Guanine Nucleotide Exchange Factor 1, CRK SH3-binding GNRP, Guanine Nucleotide-releasing Factor 2, Protein C3G, GRF2)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Immunofluorescence: 10ug/ml
Western Blot (WB)
(Western Blot analysis of RAPGEF1 expression in transfected 293T cell line by RAPGEF1 polyclonal antibody. Lane 1: RAPGEF1 transfected lysate (120.45kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of RAPGEF1 expression in transfected 293T cell line by RAPGEF1 polyclonal antibody. Lane 1: RAPGEF1 transfected lysate (120.45kD). Lane 2: Non-transfected lysate.)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to RAPGEF1 on HeLa cell. [antibody concentration 10ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to RAPGEF1 on HeLa cell. [antibody concentration 10ug/ml].)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
RAPGEF1: an adaptor-type Src homology (SH)2-containing molecule. A guanine nucleotide releasing protein for Ras. Expressed ubiquitously in human adult and fetal tissues. Several alternatively spliced transcript variants have been described.
Protein type: GEFs, Ras; GEFs; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: early endosome; cytosol; endosome
Molecular Function: Rap guanyl-nucleotide exchange factor activity; protein binding; SH3 domain binding
Biological Process: blood vessel development; cell-cell adhesion; negative regulation of protein kinase B signaling cascade; nerve growth factor receptor signaling pathway; activation of MAPKK activity; negative regulation of Ras protein signal transduction; platelet-derived growth factor receptor signaling pathway; signal transduction; Rap protein signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on RAPGEF1
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Product Notes
The RAPGEF1 rapgef1 (Catalog #AAA6004950) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAPGEF1 (Rap Guanine Nucleotide Exchange Factor 1, CRK SH3-binding GNRP, Guanine Nucleotide-releasing Factor 2, Protein C3G, GRF2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAPGEF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence and Western Blot. Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the RAPGEF1 rapgef1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGNAIEKQKP LKRSHLYPWK QDSQRSHLSS FTMKLMDKFH SPKIKRTPSK KGKPAEVSVK IPEKPVNKEA TDRFLPEGYP LPLDLEQQAV EFMSTSAVAS RSQRQKNLSW LEEKEKEVVS ALRYFKTIVD KMAIDKKVLE MLPGSASKVL EAILPLVQND PRIQHSSALS SCYSRVYQSL ANLIRWSDQV MLEGVNSEDK EMVTTVKGVI KAVLDGVKEL VRLTIEKQGR PSPTSPVKPS SPASKPDGPA ELPLTDREVE ILNKTTGMSQ STELLPDATD EEVAPPKPPL PGIRVVDNSP PPALPPKKRQ SAPSPTRVAV VAPMSRATSG SSLPVGINRQ DFDVDCYAQR RLSGGSHSYG GESPRLSPCS SIGKLSKSDE QLSSLDRDSG QCSRNTSCET LDHYDPDYEF LQQDLSNADQ IPQQTAWNLS PLPESLGESG SPFLGPPFQL PLGGHPQPDG PLAPGQQTDT PPALPEKKRR SAASQTADGS GCRVSYERHP SQYDNISGED LQSTAPIPSV PYAPFAAILP FQHGGSSAPV EFVGDFTAPE STGDPEKPPP LPEKKNKHML AYMQLLEDYS EPQPSMFYQT PQNEHIYQQK NKLLMEVYGF SDSFSGVDSV QELAPPPALP PKQRQLEPPA GKDGHPRDPS AVSGVPGKDS RDGSERAPKS PDALESAQSE EEVDELSLID HNEIMSRLTL KQEGDDGPDV RGGSGDILLV HATETDRKDL VLYCEAFLTT YRTFISPEEL IKKLQYRYEK FSPFADTFKK RVSKNTFFVL VRVVDELCLV ELTEEILKLL MELVFRLVCN GELSLARVLR KNILDKVDQK KLLRCATSSQ PLAARGVAAR PGTLHDFHSH EIAEQLTLLD AELFYKIEIP EVLLWAKEQN EEKSPNLTQF TEHFNNMSYW VRSIIMLQEK AQDRERLLLK FIKIMKHLRK LNNFNSYLAI LSALDSAPIR RLEWQKQTSE GLAEYCTLID SSSSFRAYRA ALSEVEPPCI PYLGLILQDL TFVHLGNPDY IDGKVNFSKR WQQFNILDSM RCFQQAHYDM RRNDDIINFF NDFSDHLAEE ALWELSLKIK PRNITRRKTD REEKT. It is sometimes possible for the material contained within the vial of "RAPGEF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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