ESAM recombinant protein

ESAM Protein, Rat, Recombinant (hFc Tag)

Cat. Num. AAA8123393

• Purity: >85% as determined by SDS-PAGE
• Synonyms: ESAM; ESAM Protein; Rat; Recombinant (hFc Tag); Rat ESAM/Endothelial Cell Adhesion Molecule Protein (Fc Tag); endothelial cell adhesion molecule; ESAM recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala251

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 30
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the rat ESAM (Q6AYD4) extracellular domain (Met 1-Ala 251) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ESAM recombinant protein

Background: Endothelial cell-selective adhesion molecule (ESAM) is a member of JAM family of immunoglobulin superfamily and consists of one V-type and one C2-type immunoglobulin domain, as well as a hydrophobic signal sequence, a single transmembrane region, and a cytoplasmic domain. It is specifically expressed at endothelial tight junctions and on activated platelets. ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel wall, possibly by influencing endothelial cell contacts. The adaptor protein membrane-associated guanylate kinase MAGI-1 has been identified as an intracellular binding partner of ESAM. Previous studies have indicated that ESAM regulates angiogenesis in the primary tumor growth and endothelial permeability. It suggest that ESAM has a redundant functional role in physiological angiogenesis but serves a unique and essential role in pathological angiogenic processes such as tumor growth.

References

Ishida T, et al. (2003) Targeted disruption of endothelial cell-selective adhesion molecule inhibits angiogenic processes in vitro and in vivo. J Biol Chem. 278(36): 34598-604.Wegmann F, et al. (2004) Endothelial adhesion molecule ESAM binds directly to the multidomain adaptor MAGI-1 and recruits it to cell contacts. Exp Cell Res. 300(1): 121-33.Wegmann F, et al. (2006) ESAM supports neutrophil extravasation, activation of Rho, and VEGF-induced vascular permeability. J Exp Med. 203(7): 1671-7.Hara T, et al. (2009) Endothelial cell-selective adhesion molecule regulates albuminuria in diabetic nephropathy. Microvasc Res. 77(3): 348-55.Cangara HM, et al. (2010) Role of endothelial cell-selective adhesion molecule in hematogeneous metastasis. Microvasc Res. 80(1): 133-41.

NCBI and Uniprot Product Information

• NCBI GI #: 51948462
• NCBI GeneID: 300519
• NCBI Accession #: NP_001004245.1
• NCBI GenBank Nucleotide #: NM_001004245.1
• UniProt Accession #: Q6AYD4
• Molecular Weight: 41,936 Da
• NCBI Official Full Name: endothelial cell-selective adhesion molecule
• NCBI Official Synonym Full Names: endothelial cell adhesion molecule
• NCBI Official Symbol: Esam
• NCBI Protein Information: endothelial cell-selective adhesion molecule
• UniProt Protein Name: Endothelial cell-selective adhesion molecule
• Protein Family: Endothelial cell-selective adhesion molecule
• UniProt Gene Name: Esam
• UniProt Entry Name: ESAM_RAT

Uniprot Description

ESAM: Can mediate aggregation most likely through a homophilic molecular interaction.

Protein type: Membrane protein, integral

Cellular Component: tight junction; adherens junction; integral to membrane; plasma membrane

Biological Process: cell-cell adhesion; homophilic cell adhesion