Mouse anti-Human RANBP3 Monoclonal Antibody | anti-RANBP3 antibody

RANBP3 antibody

Cat. Num. AAA200302

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: By protein-A affinity chromatography
• Synonyms: RANBP3; Monoclonal Antibody; RANBP3 antibody; Ran-binding protein 3 isoform RANBP3-b; RAN binding protein 3; anti-RANBP3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: AT12E11
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 499

• Applicable Applications for anti-RANBP3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended starting dilution is 1:500.
• Antigen Species: Human
• Immunogen: Recombinant human RANBP3 (235-445aa) purified from E. coli
• Clone Note: Anti-human RANBP3 mAb, clone AT12E11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human RANBP3 protein.
• Preparation and Storage: Can be stored at 4 degree C.; For long term storage, aliquot and store at -20 degree C.; Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The cell lysates of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RANBP3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Related Product Information for anti-RANBP3 antibody

The GTPase Ran is a small protein that belongs to the RAS protein superfamily and is found associated with the nuclear membrane. Ran GTPase is essential for mRNA processing, nuclear transport, cell cycle control, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance. Ran binding proteins (RanBPs) belong to a family of proteins that bind Ran GTPase and help to stimulate its GTPase activity. Members of the RanBP family show weak similarity to importin beta, a protein involved in the transport of proteins to the nuclear membrane. Recently it has been shown that RanBP3 function is regulated by the Ras/ERK/RSK and PI3K/Akt signaling pathway. This finding has provided a link between nuclear transport, cell signaling, and cell fate.

Product Categories/Family for anti-RANBP3 antibody

Cell Cycle

References

Englmeier L, (2001) EMBO Rep. 2 (10):926-32; Mueller L, (1998) FEBS Lett. 427 (3):330-6

NCBI and Uniprot Product Information

• NCBI GI #: 194097489
• NCBI GeneID: 8498
• NCBI Accession #: NP_015559
• NCBI GenBank Nucleotide #: NM_007320.2
• UniProt Accession #: Q9H6Z4; O60405; O75759; O75760; Q9BT47; Q9UG74; B2RAT8
• Molecular Weight: 53,411 Da
• NCBI Official Full Name: ran-binding protein 3 isoform RANBP3-b
• NCBI Official Synonym Full Names: RAN binding protein 3
• NCBI Official Symbol: RANBP3
• NCBI Protein Information: ran-binding protein 3
• UniProt Protein Name: Ran-binding protein 3
• Protein Family: Ran-binding protein
• UniProt Gene Name: RANBP3
• UniProt Synonym Gene Names: RanBP3

NCBI Description

This gene encodes a protein with a RanBD1 domain that is found in both the nucleus and cytoplasm. This protein plays a role in nuclear export as part of a heteromeric complex. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export.