Rabbit RanBP3 Polyclonal Antibody | anti-RANBP3 antibody

RanBP3, phosphorylated (Ser58) (Ran-binding Protein 3)

Cat. Num. AAA6001750

• Reactivity: Human, Monkey
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RanBP3; Polyclonal Antibody; phosphorylated (Ser58) (Ran-binding Protein 3); Anti -RanBP3; anti-RANBP3 antibody

• Host: Rabbit
• Reactivity: Human, Monkey
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of human RanBP3b when phosphorylated at Ser58 and RanBP3a when phosphorylated at corresponding residue Ser126. Species Crossreactivity: monkey.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-RANBP3 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide corresponding to residues surrounding Ser58 of human RanBP3b.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from HeLa and COS-7 cells, serum-starved overnight and either left untreated or treated with hEGF (100ng/ml), using Phospho-RanBP3 (Ser58) Antibody (upper) Rabbit mAb (lower))

Related Product Information for anti-RANBP3 antibody

RanBP3 was originally identified as RanGTP binding protein located in the nucleus and involved in the nuclear exporting process (1). It functions as a cofactor for CRM1 nuclear export by binding to CRM1, stabilizing the RanGTP-CRM1-cargo interaction and promoting complex association with nuclear pore proteins (2,3). In the absence of Ran-bound GTP, RanBP3 prevents binding of CRM1 complex to the nuclear pore complex. In addition to CRM1, RanBP3 also has been shown to bind to RanGEF-RCC1 and increase the guanine nucleotide exchange activity of RCC1 for RanGTP-CRM1-Cargo (1,4). In some cases, as with B-catenin and Smad2/3, RanBP3 binding may mediate the target protein nuclear export in a Ran-dependent, but CRM1-independent manner (5,6). RanBP3 is phosphorylated at Ser58 through the PI3K/Akt or ERK/RSK pathway. This phosphorylation is important for RanBP3 function in nuclear export, likely due to stimulation of RCC1 activity (7,8).

Product Categories/Family for anti-RANBP3 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 194097489
• NCBI GeneID: 8498
• NCBI Accession #: NP_015559.2
• NCBI GenBank Nucleotide #: NM_007320.2
• UniProt Accession #: Q9H6Z4; O60405; O75759; O75760; Q9BT47; Q9UG74; B2RAT8
• Molecular Weight: 60,210 Da
• NCBI Official Full Name: ran-binding protein 3 isoform RANBP3-b
• NCBI Official Synonym Full Names: RAN binding protein 3
• NCBI Official Symbol: RANBP3
• NCBI Protein Information: ran-binding protein 3; RAN-binding protein-3
• UniProt Protein Name: Ran-binding protein 3
• Protein Family: Ran-binding protein
• UniProt Gene Name: RANBP3
• UniProt Synonym Gene Names: RanBP3
• UniProt Entry Name: RANB3_HUMAN

NCBI Description

This gene encodes a protein with a RanBD1 domain that is found in both the nucleus and cytoplasm. This protein plays a role in nuclear export as part of a heteromeric complex. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

RANBP3: Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF- beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export. Interacts with CHC1 in a Ran-stimulated manner. Interacts with XPO1. Interacts (via its C-terminal R domain) with SMAD2 (dephosphorylated form via its MH1 and MH2 domains); the interaction results in the nuclear export of SMAD2 and termination of the TGF-beta signaling. Interacts (via its C-terminal R domain) with SMAD3 (dephosphorylated form via its MH1 domain); the interaction results in the nuclear export of SMAD3 and termination of the TGF-beta signaling. Widely expressed with high levels in testis and heart. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; Ran GTPase binding

Biological Process: protein transport; intracellular transport