Western Blot (WB)
(Western blot analysis of extracts of HeLa and RAW 264.7 cells, using Phospho-PTPN6-Y564 antibody at 1:2000 dilution. HeLa cell lysates were treated by CIP at 37 degree C for 1 hour.RAW 264.7 cell lysates were treated by CIP at 37 degree C for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit.Exposure time: 3min.)
Rabbit anti-Human, Mouse PTPN6-Y564 Polyclonal Antibody | anti-PTPN6-Y564 antibody
Phospho-PTPN6-Y564 pAb
Western Blot (WB)
(Western blot analysis of extracts of HeLa and RAW 264.7 cells, using Phospho-PTPN6-Y564 antibody at 1:2000 dilution. HeLa cell lysates were treated by CIP at 37 degree C for 1 hour.RAW 264.7 cell lysates were treated by CIP at 37 degree C for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit.Exposure time: 3min.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa and RAW 264.7 cells, using Phospho-PTPN6-Y564 antibody at 1:2000 dilution. HeLa cell lysates were treated by CIP at 37 degree C for 1 hour.RAW 264.7 cell lysates were treated by CIP at 37 degree C for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit.Exposure time: 3min.)
NCBI and Uniprot Product Information
Observed: 60kDa
NCBI Description
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
Research Articles on PTPN6-Y564
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Product Notes
The PTPN6-Y564 ptpn6 (Catalog #AAA9135640) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PTPN6-Y564 pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PTPN6-Y564 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the PTPN6-Y564 ptpn6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DVYEN. It is sometimes possible for the material contained within the vial of "PTPN6-Y564, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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