Western Blot (WB)
(Western Blot using anti-RAGE antibody 2A11 (MBS488645). Human lung lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 2A11 () at 0.03ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Rabbit anti-Human RAGE Monoclonal Antibody | anti-RAGE antibody
Anti-RAGE [2A11]
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, ELISA, Flow Cytometry, Functional Assay
Purity
Purified antibody. Protein A affinity purified
Synonyms
RAGE; Monoclonal Antibody; Anti-RAGE [2A11]; AGER; advanced glycosylation end product-specific receptor; receptor for advanced glycosylation end products; anti-RAGE antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
2A11
Specificity
2A11 is specific for the V-domain of human advanced glycosylation end product-specific receptor. It recognizes both the glycosylated and non-glycosylated forms.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-RAGE antibody
Western blot, Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA), Flow Cytometry (FC/FACS), Surface Plasmon Resonance (SPR)
Application Notes
ELISA confirmed the specificity of 2A11 for the V-domain of RAGE, with a binding affinity (Kd) of 7.6 nM. ELISA was also used to confirm that the binding of 2A11 to RAGE is glycosylation independent. In SPR experiments the Kd of 2A11 binding to RAGE was determined as 0.38 nM. Flow cytometry and immunochemistry were used to visualise the binding of 2A11 to HEK-RAGE cells.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Western Blot (WB)
(Western Blot using anti-RAGE antibody 2A11 (MBS488645). Human lung lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 2A11 () at 0.03ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-RAGE antibody 2A11 (MBS488645). Human lung lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 2A11 () at 0.03ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Immunofluorescence (IF)
(Immunofluorescence staining of A549 cells using anti-RAGE antibody (MBS488645) 2A11. Immunofluorescence analysis of paraformaldehyde fixed A549 cells stained with the chimeric rabbit IgG version of 2A11 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of A549 cells using anti-RAGE antibody (MBS488645) 2A11. Immunofluorescence analysis of paraformaldehyde fixed A549 cells stained with the chimeric rabbit IgG version of 2A11 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-RAGE antibody 2A11 (MBS488645). A549 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of 2A11 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-RAGE antibody 2A11 (MBS488645). A549 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of 2A11 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-RAGE antibody
Recombinant monoclonal antibody to RAGE. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2A11.
Human RAGE protein.
Human RAGE protein.
Product Categories/Family for anti-RAGE antibody
References
Jyoti, 2012. Development of new antibody based theranostic agents targeting the receptor for advanced glycation end-product (RAGE). Dissertation Submitted to the Graduate Faculty of the North Dakota State University of Agriculture and Applied Science
Note on publication: Describes the characterisation of 2A11 through ELISA, SPR, FC, and IH experiments.
Note on publication: Describes the characterisation of 2A11 through ELISA, SPR, FC, and IH experiments.
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The RAGE (Catalog #AAA488645) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RAGE [2A11] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAGE can be used in a range of immunoassay formats including, but not limited to, Western blot, Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA), Flow Cytometry (FC/FACS), Surface Plasmon Resonance (SPR). ELISA confirmed the specificity of 2A11 for the V-domain of RAGE, with a binding affinity (Kd) of 7.6 nM. ELISA was also used to confirm that the binding of 2A11 to RAGE is glycosylation independent. In SPR experiments the Kd of 2A11 binding to RAGE was determined as 0.38 nM. Flow cytometry and immunochemistry were used to visualise the binding of 2A11 to HEK-RAGE cells. Researchers should empirically determine the suitability of the RAGE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAGE, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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