Rat anti-Human Raf1 Monoclonal Antibody | anti-RAF1 antibody

Raf1, phosphorylated (Ser338, Tyr341)

Cat. Num. AAA609116

• Gene Names: RAF1; NS5; CRAF; Raf-1; c-Raf
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Raf1; Monoclonal Antibody; phosphorylated (Ser338; Tyr341); Anti -Raf1; anti-RAF1 antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5G203
• Specificity: Recognizes Raf1. Binds to phosphorylated serine residue at aa338. It does not bind to unactivated Raf- 1 or Raf-1 phosphorylated at Y341 and S339. Species Crossreactivity: Human.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
• Sequence: GQRDS*SYY*WEIEAS.

• Applicable Applications for anti-RAF1 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Immunohistology: (Formalin/paraffin) (Use Ab at 2-4ug/ml for 30 minute at RT) Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
• Immunogen: Synthetic peptide (GQRDS*SYY*WEIEAS)
• Cellular Localization: Nuclear
• Positive Control: Tonsil
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-RAF1 antibody

Raf1 is an important mediator of signal transduction pathway. It is activated through activation by Src and protein kinase C. It further phosphorylates Mek1 and cdc25. It can bind to GTP-bound Ras through N-terminal domain. It also interacts with hsp90 and 14-3-3 family of proteins.

Product Categories/Family for anti-RAF1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 496091
• NCBI GeneID: 5894
• UniProt Accession #: P04049; Q15278; Q9UC20; B0LPH8; B2R5N3
• Molecular Weight: 73,052 Da
• NCBI Official Full Name: RAF1
• NCBI Official Synonym Full Names: v-raf-1 murine leukemia viral oncogene homolog 1
• NCBI Official Symbol: RAF1
• NCBI Official Synonym Symbols: NS5; CRAF; Raf-1; c-Raf
• NCBI Protein Information: RAF proto-oncogene serine/threonine-protein kinase; Oncogene RAF1; OTTHUMP00000160218; OTTHUMP00000207813; OTTHUMP00000209389; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase
• UniProt Protein Name: RAF proto-oncogene serine/threonine-protein kinase
• Protein Family: Rik1-associated factor
• UniProt Gene Name: RAF1
• UniProt Synonym Gene Names: RAF
• UniProt Entry Name: RAF1_HUMAN

NCBI Description

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq]

Uniprot Description

RAF1: a proto-oncogenic TKL kinase of the RAF family. Functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate and activate MEK1/2, which in turn phosphorylate and activate ERK1/2. Acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MEK1/2) and the extracellular signal-regulated kinases (ERK1/2). RAF1, subsequent to phosphorylation by PAK1, phosphorylates BAD (Bcl2-antagonist of cell death) at S75. Phosphorylates adenylyl cyclases ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2 (cardiac muscle troponin T). Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (ASK1 and MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Protein type: Kinase, protein; Oncoprotein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; TKL group; RAF family

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: Golgi apparatus; mitochondrial outer membrane; cytoplasm; plasma membrane; pseudopodium; cytosol; nucleus

Molecular Function: small GTPase binding; protein serine/threonine kinase activity; identical protein binding; protein binding; MAP kinase kinase kinase activity; protein heterodimerization activity; mitogen-activated protein kinase kinase binding; metal ion binding; ATP binding; protein kinase activity

Biological Process: axon guidance; wound healing; activation of MAPKK activity; nerve growth factor receptor signaling pathway; somatic stem cell maintenance; apoptosis; heart development; intermediate filament cytoskeleton organization and biogenesis; negative regulation of caspase activity; signal transduction; protein amino acid phosphorylation; regulation of apoptosis; synaptic transmission; negative regulation of cell proliferation; regulation of cell differentiation; small GTPase mediated signal transduction; transmembrane transport; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; adenylate cyclase activation; MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; cell proliferation; Ras protein signal transduction; response to hypoxia; regulation of Rho protein signal transduction; insulin receptor signaling pathway; innate immune response; vascular endothelial growth factor receptor signaling pathway; blood coagulation; negative regulation of protein complex assembly; negative regulation of apoptosis

Disease: Cardiomyopathy, Dilated, 1nn; Noonan Syndrome 5; Leopard Syndrome 2