SUMO3 recombinant protein

SUMO3 recombinant protein

Cat. Num. AAA515106

• Gene Names: SUMO3; SMT3A; Smt3B; SMT3H1; SUMO-3
• Applications: Western Blot
• Synonyms: SUMO3; SUMO3 recombinant protein; SMT3A; SMT3H1; SUMO-3; Smt3B

• Host: E Coli
• Form/Format: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Sequence Positions: 1-92
• Sequence Length: 1831

• Applicable Applications for SUMO3 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: His tag
• Expression System: E.coli
• Source Note: Recombinant human SUMO3 (1-92) was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for SUMO3 recombinant protein

Recombinant human SUMO3 (1-92) was expressed in E. coli cells using an N-terminal His tag.

Scientific Background: SUMO3 is a member of the SUMO (small ubiquitin-like modifier) protein family which functions in a manner similar to ubiquitin. SUMO3 is bound to target proteins as part of a post-translational modification system and modifies numerous cellular proteins and affect their metabolism and function. SUMO3 is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability (1). SUMO3 predominantly detected in the cytoplasm (2).

Product Categories/Family for SUMO3 recombinant protein

Ubiquitin Proteins; Signaling Proteins - Ubiquitin Proteins

References

1. Su, H.-L. et.al: Molecular features of human ubiquitin-like SUMO genes and their encoded proteins. Gene 296: 65-73, 2002.
2. Lapenta, V.et.al: SMT3A, a human homologue of the S. cerevisiae SMT3 gene, maps to chromosome 21qter and defines a novel gene family. Genomics 40: 362-366, 1997.

NCBI and Uniprot Product Information

• NCBI GI #: 48928057
• NCBI GeneID: 6612
• NCBI Accession #: NP_008867.2
• NCBI GenBank Nucleotide #: NM_006936
• UniProt Accession #: P55854; Q53HI9; Q6FGD4; Q9BWR4; B2R5X4
• Molecular Weight: ~17 kDa
• NCBI Official Full Name: Homo sapiens small ubiquitin-like modifier 3 (SUMO3), mRNA
• NCBI Official Synonym Full Names: small ubiquitin-like modifier 3
• NCBI Official Symbol: SUMO3
• NCBI Official Synonym Symbols: SMT3A; Smt3B; SMT3H1; SUMO-3
• NCBI Protein Information: small ubiquitin-related modifier 3; SUMO-2; SMT3 homolog 1; ubiquitin-like protein SMT3A; ubiquitin-like protein SMT3B; SMT3 suppressor of mif two 3 homolog 1; SMT3 suppressor of mif two 3 homolog 3
• UniProt Protein Name: Small ubiquitin-related modifier 3
• Protein Family: Small ubiquitin-related modifier
• UniProt Gene Name: SUMO3
• UniProt Synonym Gene Names: SMT3B; SMT3H1; SUMO-3; Smt3B
• UniProt Entry Name: SUMO3_HUMAN

NCBI Description

SUMO proteins, such as SUMO3, and ubiquitin (see MIM 191339) posttranslationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability (Su and Li, 2002 [PubMed 12383504]).[supplied by OMIM, Mar 2008]

Uniprot Description

Function: Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. Ref.9 Ref.15

Subunit structure: Covalently attached to a number of proteins

By similarity. Interacts with USP25 (via ts SIM domain); the interaction sumoylates USP25 and inhibits its ubiquitin hydrolyzing activity. Interacts with SAE2 and UBE2I. Ref.9 Ref.11 Ref.15

Subcellular location: Cytoplasm Ref.10.

Tissue specificity: Expressed predominantly in liver. Ref.13

Post-translational modification: Polymeric chains can be formed through Lys-11 cross-linking.Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.

Sequence similarities: Belongs to the ubiquitin family. SUMO subfamily.Contains 1 ubiquitin-like domain.

Caution: Frequently wrongly assigned as SMT3A in many databases and publications. However, according to initial nomenclature, SUMO3 corresponds to SMT3B (Ref.8).