Western Blot (WB)
(Western Blot analysis of RAF1 expression in transfected 293T cell line by RAF1 monoclonal antibody. Lane 1: RAF1 transfected lysate (73.1kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human RAF1 Monoclonal Antibody | anti-RAF1 antibody
RAF1 (RAF Proto-oncogene Serine/Threonine-protein Kinase, Proto-oncogene c-RAF, Raf-1, C-RAF, cRaf, RAF) (Biotin)
Western Blot (WB)
(Western Blot analysis of RAF1 expression in transfected 293T cell line by RAF1 monoclonal antibody. Lane 1: RAF1 transfected lysate (73.1kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of RAF1 expression in transfected 293T cell line by RAF1 monoclonal antibody. Lane 1: RAF1 transfected lysate (73.1kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western blot analysis of RAF1 over-expressed 293 cell line, cotransfected with RAF1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAF1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Western Blot (WB)
(Western blot analysis of RAF1 over-expressed 293 cell line, cotransfected with RAF1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAF1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Testing Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between BAD and RAF1. HeLa cells were stained with BAD rabbit purified polyclonal 1:1200 and RAF1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Testing Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between BAD and RAF1. HeLa cells were stained with BAD rabbit purified polyclonal 1:1200 and RAF1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Testing Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between PDGFRB and RAF1. Huh7 cells were stained with PDGFRB rabbit purified polyclonal 1:600 and RAF1 mouse monoclonal antibody 1:100. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Testing Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between PDGFRB and RAF1. Huh7 cells were stained with PDGFRB rabbit purified polyclonal 1:600 and RAF1 mouse monoclonal antibody 1:100. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
NCBI and Uniprot Product Information
NCBI Description
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]
Research Articles on RAF1
Similar Products
Product Notes
The RAF1 (Catalog #AAA6143905) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAF1 (RAF Proto-oncogene Serine/Threonine-protein Kinase, Proto-oncogene c-RAF, Raf-1, C-RAF, cRaf, RAF) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAF1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RAF1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
