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Western Blot (WB) (Western blot analysis of Radixin on different lysates using anti-Radixin antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: HepG2)

Rabbit Radixin Monoclonal Antibody | anti-RDX antibody

Radixin Antibody

Gene Names
RDX; DFNB24
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Radixin; Monoclonal Antibody; Radixin Antibody; CB567 antibody; CG12537 antibody; DFNB24 antibody; ESP10 antibody; Hh-induced MATH and BTB domain-containing protein antibody; HIB antibody; Moesin-B antibody; Protein roadkill antibody; RADI_HUMAN antibody; Radixin antibody; RDX antibody; anti-RDX antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SC06-14
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RDX antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Radixin on different lysates using anti-Radixin antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: HepG2)

Western Blot (WB) (Western blot analysis of Radixin on different lysates using anti-Radixin antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: HepG2)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Radixin antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Radixin antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Radixin antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Radixin antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Radixin antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Radixin antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Radixin antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Radixin antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining Radixin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining Radixin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Hela cells with Radixin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of Hela cells with Radixin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-RDX antibody
Ezrin, Moesin and Radixin belong to a family of highly homologous actin-associated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70% homology with Ezrin and are co-expressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation.
Product Categories/Family for anti-RDX antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
68,564 Da
NCBI Official Full Name
radixin isoform 3
NCBI Official Synonym Full Names
radixin
NCBI Official Symbol
RDX
NCBI Official Synonym Symbols
DFNB24
NCBI Protein Information
radixin
UniProt Protein Name
Radixin
Protein Family
UniProt Gene Name
RDX
UniProt Entry Name
RADI_HUMAN

NCBI Description

Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Radixin: a cytoskeletal protein of the ERM family which includes ezrin and moiesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. May play a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: extracellular space; focal adhesion; T-tubule; ruffle; stereocilium; extrinsic to membrane; cytoskeleton; lamellipodium; apical part of cell; cytoplasm; plasma membrane; filopodium; cleavage furrow

Molecular Function: protein binding; actin binding

Biological Process: microvillus biogenesis; apical protein localization; actin filament capping

Disease: Deafness, Autosomal Recessive 24

Research Articles on RDX

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Product Notes

The RDX rdx (Catalog #AAA9435747) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Radixin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Radixin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the RDX rdx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Radixin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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