Rabbit anti-Human RNF34 Polyclonal Antibody | anti-RNF34 antibody

Rabbit Polyclonal to Human RNF34

Cat. Num. AAA240834

• Gene Names: RNF34; RFI; RIF; RIFF; hRFI; CARP1; CARP-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: RNF34; Polyclonal Antibody; Rabbit Polyclonal to Human RNF34; Anti-RNF34 Antibody (aa1-373) IHC-plus; CARP1; Caspase regulator CARP1; CARP-1; FYVE-RING finger protein Momo; HRFI; RFI; RIF; RIFF; RING finger protein 34; RING finger protein RIFF; Human RNF34; anti-RNF34 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Amino acids 1-373 of human RNF34 protein.
• Purity/Purification: Protein A Purified
• Form/Format: Lyophilized.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 372

• Applicable Applications for anti-RNF34 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:4000 - 1:20000), IHC-P (10 ug/ml), WB (1:500 - 1:3000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Recombinant human RNF34.
• Immunogen Type: Recombinant protein
• Immunogen: RNF34 antibody was raised against recombinant human RNF34.
• Antigen Modification: aa1-373
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-RNF34 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Anti-RNF34 Antibody - Western Blot. Western blot of Protein A Purified anti-RNF34 antibody shows detection of human RNF34 (arrowhead) in lysate. Lanes corresponding to empty vector 293T cell lysate (mock, left); RNF34 transfected lysate (middle) and RFFL transfected lysate (right), are shown using 20 ul of lysate per lane. Lysates were prepared from equivalent numbers of cells. Data presented demonstrate that this reagent is specific for RNF34. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1000 using 5% BLOTTO, 0.1% Tween-20 in PBS as the diluent. Incubation occurred for 1 h at room temperature. Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.)

NCBI and Uniprot Product Information

• NCBI GI #: 37595537
• NCBI GeneID: 80196
• NCBI Accession #: NP_079402.2
• NCBI GenBank Nucleotide #: NM_025126.3
• UniProt Accession #: Q969K3; Q8NG47; Q9H6W8; B7Z933
• Molecular Weight: 41,797 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF34 isoform 2
• NCBI Official Synonym Full Names: ring finger protein 34, E3 ubiquitin protein ligase
• NCBI Official Symbol: RNF34
• NCBI Official Synonym Symbols: RFI; RIF; RIFF; hRFI; CARP1; CARP-1
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF34; caspase regulator CARP1; RING finger protein RIFF; FYVE-RING finger protein MOMO; caspases-8 and -10-associated RING finger protein 1; human RING finger homologous to inhibitor of apoptosis protein
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF34
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF34
• UniProt Entry Name: RNF34_HUMAN

NCBI Description

The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]

Uniprot Description

RNF34: Has E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol 5-phosphate and phosphatidylinositol 3- phosphate. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ubiquitin ligase; Ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cytoplasm; plasma membrane; endomembrane system; nuclear speck; nucleus; cytosol

Molecular Function: protein binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination