Cytoglobin Recombinant Protein | CYGB recombinant protein

Recombinant Cytoglobin (CYGB)

Cat. Num. AAA2030777

• Gene Names: CYGB; HGB; STAP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Cytoglobin; Recombinant Cytoglobin (CYGB); CYGB recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MEKVPGDMEI ERRERNEELS EAERKAVQAT WARLYANCED VGVAILVRFFVNFPSAKQYF SQFKHMEDPL EMERSPQLRK HACRVMGALN TVVENLHDPDKVSSVLALVG KAHALKHKVE PMYFKILSGV ILDVIAEEFA NDFPVETQKAWTKLRGLIYS HVTAAYKEVG WVQQVPNTTT LPATLPSSGP
• Sequence Length: 190

• Applicable Applications for CYGB recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 25.2kDa
• Accurate Molecular Mass (KD): 28kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Met1~Pro190
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 19549331
• NCBI GeneID: 114757
• NCBI Accession #: NP_599030.1
• NCBI GenBank Nucleotide #: NM_134268.4
• Molecular Weight: 21,405 Da
• NCBI Official Full Name: cytoglobin
• NCBI Official Synonym Full Names: cytoglobin
• NCBI Official Symbol: CYGB
• NCBI Official Synonym Symbols: HGB; STAP
• NCBI Protein Information: cytoglobin
• UniProt Protein Name: Cytoglobin
• Protein Family: Cytoglobin
• UniProt Gene Name: CYGB
• UniProt Synonym Gene Names: STAP; HGb
• UniProt Entry Name: CYGB_HUMAN

NCBI Description

This gene encodes a globin protein found in vertebrate cells. The encoded protein is described as a hexacoordinate hemoglobin which binds ligand differently from the pentacoordinate hemoglobins involved in oxygen transport, and may be involved in protection during oxidative stress. This gene is located on chromosome 17 in the same region as a retinal gene which is mutated in progressive rod-cone degeneration, but in the opposite orientation. [provided by RefSeq, Jan 2012]

Uniprot Description

CYGB: May have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer. Belongs to the globin family.

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: neuron projection; cell soma; cytoplasm

Molecular Function: peroxidase activity; iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: oxygen transport; response to oxidative stress