Western Blot (WB)
(Western blot detection of Rad50 in K562,Hela cell lysates using Rad50 Rabbit mAb(1:1000 diluted). Predicted band size:154kDa. Observed band size:154kDa.)
Rabbit anti-Human, Mouse Rad50 Monoclonal Antibody | anti-RAD50 antibody
Rad50 Rabbit mAb
Gene Names
RAD50; NBSLD; RAD502; hRad50
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Affinity purified
Synonyms
Rad50; Monoclonal Antibody; Rad50 Rabbit mAb; NBSLD; RAD502; hRad50; anti-RAD50 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
R05-5H6
Specificity
Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-RAD50 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:2000
ICC/IF: 1:20
ICC/IF: 1:20
Immunogen
A synthetic peptide of human Rad50
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot detection of Rad50 in K562,Hela cell lysates using Rad50 Rabbit mAb(1:1000 diluted). Predicted band size:154kDa. Observed band size:154kDa.)
Western Blot (WB)
(Western blot detection of Rad50 in K562,Hela cell lysates using Rad50 Rabbit mAb(1:1000 diluted). Predicted band size:154kDa. Observed band size:154kDa.)
Related Product Information for anti-RAD50 antibody
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:11741547, PubMed:9590181, PubMed:9705271, PubMed:9651580). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
150kDa
NCBI Official Full Name
DNA repair protein RAD50
NCBI Official Synonym Full Names
RAD50 homolog (S. cerevisiae)
NCBI Official Symbol
RAD50
NCBI Official Synonym Symbols
NBSLD; RAD502; hRad50
NCBI Protein Information
DNA repair protein RAD50
UniProt Protein Name
DNA repair protein RAD50
Protein Family
UniProt Gene Name
RAD50
UniProt Synonym Gene Names
hRAD50
UniProt Entry Name
RAD50_HUMAN
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Product Notes
The RAD50 rad50 (Catalog #AAA4752412) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rad50 Rabbit mAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rad50 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:2000 ICC/IF: 1:20. Researchers should empirically determine the suitability of the RAD50 rad50 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rad50, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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