Immunohistochemistry (IHC)
(Figure: Immunohistochemical staining of endogenous human RANKL in different human tissues (paraffin sections) using MAb to RANKL (human)(Ranky-1) . Method: Different human tissues (A: Duodenum; B: Small intestine) were stained with MAb to RANKL (human) (Ranky-1) by standard immunohistochemistry. Pictures courtesy of Human Protein Atlas (www.proteinatlas.org).)
anti-Human RANKL Monoclonal Antibody | anti-ODF; TRANCE; OPGL; TNFSF 11; CD254 antibody
RANKL (human) monoclonal antibody (Ranky-1)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Does not work for Flow Cytometry or Immunoprecipitation. Does not block RANK-RANKL interaction.
Short term: 4 degree C
Avoid freeze/thaw cycles.
Immunohistochemistry (IHC)
(Figure: Immunohistochemical staining of endogenous human RANKL in different human tissues (paraffin sections) using MAb to RANKL (human)(Ranky-1) . Method: Different human tissues (A: Duodenum; B: Small intestine) were stained with MAb to RANKL (human) (Ranky-1) by standard immunohistochemistry. Pictures courtesy of Human Protein Atlas (www.proteinatlas.org).)
Immunohistochemistry (IHC)
(Figure: Immunohistochemical staining of endogenous human RANKL in different human tissues (paraffin sections) using MAb to RANKL (human)(Ranky-1) . Method: Different human tissues (A: Duodenum; B: Small intestine) were stained with MAb to RANKL (human) (Ranky-1) by standard immunohistochemistry. Pictures courtesy of Human Protein Atlas (www.proteinatlas.org).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]
Uniprot Description
TNFSF11: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Homotrimer. Up-regulated by T-cell receptor stimulation. Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 13q14
Cellular Component: cytoplasm; extracellular region; extracellular space; integral to plasma membrane; plasma membrane
Molecular Function: cytokine activity; tumor necrosis factor receptor binding; tumor necrosis factor receptor superfamily binding
Biological Process: activation of JNK activity; activation of NF-kappaB transcription factor; bone resorption; calcium ion homeostasis; cytokine and chemokine mediated signaling pathway; immune response; mammary gland epithelial cell proliferation; monocyte chemotaxis; organ morphogenesis; ossification; osteoclast differentiation; positive regulation of bone resorption; positive regulation of corticotropin-releasing hormone secretion; positive regulation of homotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of MAP kinase activity; positive regulation of osteoclast differentiation; positive regulation of protein kinase B signaling cascade; positive regulation of T cell activation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; protein homooligomerization; tumor necrosis factor-mediated signaling pathway
Disease: Osteopetrosis, Autosomal Recessive 2
Research Articles on ODF; TRANCE; OPGL; TNFSF 11; CD254
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Product Notes
The ODF; TRANCE; OPGL; TNFSF 11; CD254 tnfsf11 (Catalog #AAA565064) is an Antibody produced from Rat; Purified from concentrated hybridoma tissue culture supernatant. and is intended for research purposes only. The product is available for immediate purchase. The RANKL (human) monoclonal antibody (Ranky-1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RANKL can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA),Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ELISA (capture) Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Does not work for Flow Cytometry or Immunoprecipitation. Does not block RANK-RANKL interaction. Researchers should empirically determine the suitability of the ODF; TRANCE; OPGL; TNFSF 11; CD254 tnfsf11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RANKL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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