Rabbit anti-Human PKP2 Polyclonal Antibody | anti-PKP2 antibody

PKP2, CT (PKP2, Plakophilin-2) (AP)

Cat. Num. AAA6334476

• Gene Names: PKP2; ARVD9
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PKP2; Polyclonal Antibody; CT (PKP2; Plakophilin-2) (AP); Plakophilin-2; anti-PKP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PKP2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PKP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 837-866 amino acids from the C-terminal region of human PKP2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PKP2 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PKP2 antibody detected the PKP2 protein (arrow).)

Immunohistochemistry (IHC)

(PKP2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PKP2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PKP2 antibody

This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This gene product may regulate the signaling activity of beta-catenin. Two alternately spliced transcripts encoding two protein isoforms have been identified. A processed pseudogene with high similarity to this locus has been mapped to chromosome 12p13.

Product Categories/Family for anti-PKP2 antibody

Antibodies; Plakophilin

References

Fressart, V., et al. Europace 12(6):861-868(2010). Cox, M.G., et al. Circ Arrhythm Electrophysiol 3(2):126-133(2010). Christensen, A.H., et al. Cardiology 115(2):148-154(2010). den Haan, A.D., et al. Circ Cardiovasc Genet 2(5):428-435(2009). Bhuiyan, Z.A., et al. Circ Cardiovasc Genet 2(5):418-427(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 148664226
• NCBI GeneID: 5318
• NCBI Accession #: NP_004563.2
• NCBI GenBank Nucleotide #: NM_004572.3
• UniProt Accession #: Q99959; Q4VC01; Q99960; A0AV37; D3DUW9
• Molecular Weight: 97,415 Da
• NCBI Official Full Name: plakophilin-2 isoform 2b
• NCBI Official Synonym Full Names: plakophilin 2
• NCBI Official Symbol: PKP2
• NCBI Official Synonym Symbols: ARVD9
• NCBI Protein Information: plakophilin-2
• UniProt Protein Name: Plakophilin-2
• Protein Family: Plakophilin
• UniProt Gene Name: PKP2
• UniProt Entry Name: PKP2_HUMAN

NCBI Description

This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This gene product may regulate the signaling activity of beta-catenin. Two alternately spliced transcripts encoding two protein isoforms have been identified. A processed pseudogene with high similarity to this locus has been mapped to chromosome 12p13. [provided by RefSeq, Jul 2008]

Uniprot Description

plakophilin 2: May play a role in junctional plaques. Defects in PKP2 are the cause of familial arrhythmogenic right ventricular dysplasia type 9 (ARVD9); also known as arrhythmogenic right ventricular cardiomyopathy 9 (ARVC9). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. Belongs to the beta-catenin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12p11

Cellular Component: nucleoplasm; desmosome; adherens junction; integral to membrane; plasma membrane; intermediate filament; intercellular junction; cell junction; nucleus

Molecular Function: protein binding; sodium channel regulator activity; protein kinase C binding; intermediate filament binding; protein complex scaffold

Biological Process: negative regulation of cell proliferation; cell-cell adhesion; gap junction assembly; heart development; lipid homeostasis; ventricular cardiac muscle morphogenesis; intermediate filament bundle assembly; negative regulation of cell migration; maintenance of organ identity

Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 9