Rabbit Rab4A Monoclonal Antibody | anti-Rab4A antibody

Rab4A Rabbit Monoclonal Antibody

Cat. Num. AAA3014065

• Gene Names: RAB4A; RAB4; HRES1; HRES-1; HRES-1/RAB4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A affinity purified
• Synonyms: Rab4A; Monoclonal Antibody; Rab4A Rabbit Monoclonal Antibody; Ras-related protein Rab-4A; RAB4A; RAB4; anti-Rab4A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This antibody detects endogenous levels of Rab4A and does not cross-react with related proteins.
• Purity/Purification: Protein A affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 113

• Applicable Applications for anti-Rab4A antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; ICC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant antibody.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Rab4A Rabbit mAb at 1:500 dilutionLane1:MCF-7 whole cell lysateLane2:293T whole cell lysate)

Immunocytochemistry (ICC)

(ICC staining Rab4A in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Related Product Information for anti-Rab4A antibody

The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab superfamilies, exhibits 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possiblity that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab family have been identified, each of which is found at a particular stage of a membrane transport pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 433660831
• NCBI GeneID: 5867
• NCBI Accession #: NP_001258927.1
• NCBI GenBank Nucleotide #: NP_001258927.1
• UniProt Accession #: P20338
• Molecular Weight: ~ 24kDa
• NCBI Official Full Name: ras-related protein Rab-4A isoform 2
• NCBI Official Synonym Full Names: RAB4A, member RAS oncogene family
• NCBI Official Symbol: RAB4A
• NCBI Official Synonym Symbols: RAB4; HRES1; HRES-1; HRES-1/RAB4
• NCBI Protein Information: ras-related protein Rab-4A
• UniProt Protein Name: Ras-related protein Rab-4A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB4A
• UniProt Synonym Gene Names: RAB4
• UniProt Entry Name: RAB4A_HUMAN

NCBI Description

This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

Rab4: a member of the RAB family of RAS-related GTP-binding proteins, important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. Mutations in this protein cause Charcot-Marie-Tooth type 2B neuropathy.

Protein type: G protein; G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 1q42-q43

Cellular Component: membrane; perinuclear region of cytoplasm; endosome; vesicle

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; protein transporter activity

Biological Process: antigen processing and presentation; intracellular protein transport; metabolic process; regulation of endocytosis; Rab protein signal transduction