Mouse anti-Human RAB8A / RAB8 Monoclonal Antibody | anti-RAB8A antibody

Mouse Monoclonal [clone 3G1] (IgG1,k) to Human RAB8A / RAB8

Cat. Num. AAA249043

• Gene Names: RAB8A; MEL; RAB8
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: RAB8A / RAB8; Monoclonal Antibody; Mouse Monoclonal [clone 3G1] (IgG1; k) to Human RAB8A / RAB8; Anti-RAB8A / RAB8 Antibody (clone 3G1) IHC-plus; RAB8A; RAB8; Ras-related protein rab-8; Oncogene c-mel; Ras-associated protein RAB8; Ras-related protein Rab-8A; MEL; Human RAB8A; anti-RAB8A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3G1
• Specificity: Human RAB8A
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4.
• Concentration: 0.45 mg/ml (varies by lot)
• Sequence Length: 207

• Applicable Applications for anti-RAB8A antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: RAB8A (AAH02977, 108 a.a. ~ 207 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: RAB8A / RAB8 antibody was raised against rAB8A (AAH02977, 108 a.a. ~ 207 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-RAB8A / RAB8 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(RAB8A monoclonal antibody (M02), clone 3G1 Western blot of RAB8A expression in HeLa NE.)

ELISA (EIA)

(Detection limit for recombinant GST tagged RAB8A is approximately 0.1 ng/ml as a capture antibody.)

Product Categories/Family for anti-RAB8A antibody

Family: Ras GTPase superfamily IPR001806

NCBI and Uniprot Product Information

• NCBI GI #: 16933567
• NCBI GeneID: 4218
• NCBI Accession #: NP_005361.2
• NCBI GenBank Nucleotide #: NM_005370.4
• UniProt Accession #: P61006; P24407; Q6FHV5; B4DEK7
• Molecular Weight: 23,564 Da
• NCBI Official Full Name: ras-related protein Rab-8A
• NCBI Official Synonym Full Names: RAB8A, member RAS oncogene family
• NCBI Official Symbol: RAB8A
• NCBI Official Synonym Symbols: MEL; RAB8
• NCBI Protein Information: ras-related protein Rab-8A; oncogene c-mel; ras-associated protein RAB8; mel transforming oncogene (RAB8 homolog); mel transforming oncogene (derived from cell line NK14); mel transforming oncogene (derived from cell line NK14)- RAB8 homolog
• UniProt Protein Name: Ras-related protein Rab-8A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB8A
• UniProt Synonym Gene Names: MEL; RAB8
• UniProt Entry Name: RAB8A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008]

Uniprot Description

RAB8A: May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO/C1orf88. Interacts with BIRC6/bruce. Interacts with OCRL. Belongs to the small GTPase superfamily. Rab family.

Protein type: Oncoprotein; Motility/polarity/chemotaxis; G protein; G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: centrosome; cytoplasmic vesicle membrane; secretory granule membrane; nonmotile primary cilium; recycling endosome membrane; dendrite; postsynaptic density; phagocytic vesicle; cytosol; cilium; Golgi membrane; nucleoplasm; phagocytic vesicle membrane; synaptic vesicle; trans-Golgi network transport vesicle; cell soma; nucleolus; plasma membrane; nucleus

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; myosin V binding; protein kinase binding; Rab GTPase binding

Biological Process: regulation of long-term neuronal synaptic plasticity; metabolic process; organelle organization and biogenesis; protein secretion; regulation of protein transport; regulation of exocytosis; intracellular protein transport; synaptic vesicle exocytosis; Golgi vesicle fusion to target membrane; axonogenesis; cellular response to insulin stimulus; cilium biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; Rab protein signal transduction; vesicle docking during exocytosis