Human Interleukin-23 Receptor ELISA Kit | IL23R elisa kit

Human Interleukin-23 Receptor ELISA Kit

• Reactivity: Human
• Synonyms: Interleukin-23 Receptor; Human Interleukin-23 Receptor ELISA Kit; IL23R elisa kit

• Reactivity: Human
• Sequence Length: 629

• Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05ng/ml-30ng/ml
• Sensitivity: 0.028ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL23R elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Interleukin 8 Receptor Beta (also known as IL-8RB) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human IL-8RB antibody. IL-8RB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human IL-8RB Antibody is added and binds to IL-8RB in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-8RB antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human IL-8RB. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 24430212
• NCBI GeneID: 149233
• NCBI Accession #: NP_653302.2
• NCBI GenBank Nucleotide #: NM_144701.2
• UniProt Accession #: Q5VWK5
• NCBI Official Full Name: interleukin-23 receptor
• NCBI Official Synonym Full Names: interleukin 23 receptor
• NCBI Official Symbol: IL23R
• NCBI Protein Information: interleukin-23 receptor
• UniProt Protein Name: Interleukin-23 receptor
• Protein Family: Interleukin-23 receptor
• UniProt Gene Name: IL23R
• UniProt Synonym Gene Names: IL-23 receptor; IL-23R
• UniProt Entry Name: IL23R_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner. [provided by RefSeq, Jul 2008]

Uniprot Description

IL23R: Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis. Genetic variations in IL23R are associated with inflammatory bowel disease type 17 (IBD17). IBD17 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Genetic variations in IL23R are a cause of susceptibility to psoriasis type 7 (PSORS7). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Belongs to the type I cytokine receptor family. Type 2 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: receptor complex

Molecular Function: interleukin-23 binding; interleukin-23 receptor activity; interleukin-12 receptor binding

Biological Process: positive regulation of memory T cell differentiation; positive regulation of interleukin-17 production; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of NK T cell activation; positive regulation of natural killer cell proliferation; negative regulation of interleukin-10 production; positive regulation of T-helper 1 type immune response; positive regulation of osteoclast differentiation; positive regulation of interleukin-12 production; positive regulation of T cell mediated cytotoxicity; positive regulation of tyrosine phosphorylation of Stat4 protein; response to lipopolysaccharide; positive regulation of activated T cell proliferation; defense response to Gram-negative bacterium; regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of interferon-gamma production; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of T cell proliferation; positive regulation of defense response to virus by host; inflammatory response

Disease: Inflammatory Bowel Disease 17; Psoriasis 7, Susceptibility To