Mouse RAB5A Monoclonal Antibody | anti-RAB5A antibody

Mouse Anti Human RAB5A

Cat. Num. AAA140308

• Gene Names: RAB5A; RAB5
• Applications: ELISA, Western Blot
• Purity: RAB5A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: RAB5A; Monoclonal Antibody; Mouse Anti Human RAB5A; RAB5A Antibody; Mouse Anti Human; Ras-related protein Rab-5A; RAB5; anti-RAB5A antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b heavy chain and kappa light chain
• Clone Number: P3A4AT
• Purity/Purification: RAB5A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 215

• Applicable Applications for anti-RAB5A antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: RAB5A antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000.Recommended starting dilution is 1:500.
• Immunogen: Anti-human RAB5A mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human RAB5A amino acids 1-215 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-RAB5A antibody

Introduction: RAB5A is a member of RAS oncogene family. The small GTPase, RAB5A is a crucial regulatory component of the endocytic pathway. Activation of RAB5A is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex). RAB5A is required for the fusion of plasma membranes and early endosomes.

Product Categories/Family for anti-RAB5A antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 19923262
• NCBI GeneID: 5868
• NCBI Accession #: NP_004153.2
• NCBI GenBank Nucleotide #: NM_004162.4
• Molecular Weight: 22,178 Da
• NCBI Official Full Name: ras-related protein Rab-5A isoform 1
• NCBI Official Synonym Full Names: RAB5A, member RAS oncogene family
• NCBI Official Symbol: RAB5A
• NCBI Official Synonym Symbols: RAB5
• NCBI Protein Information: ras-related protein Rab-5A; RAS-associated protein RAB5A
• UniProt Protein Name: Ras-related protein Rab-5A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB5A
• UniProt Synonym Gene Names: RAB5
• UniProt Entry Name: RAB5A_HUMAN

Uniprot Description

RAB5A: Required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, RABEP1, SUN2, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein; Motility/polarity/chemotaxis; G protein, monomeric, Rab; G protein, monomeric

Chromosomal Location of Human Ortholog: 3p24-p22

Cellular Component: zymogen granule membrane; protein complex; dendrite; early endosome; terminal button; phagocytic vesicle; cytosol; lipid raft; actin cytoskeleton; ruffle; recycling endosome; synaptic vesicle; cell soma; axon; endocytic vesicle; perinuclear region of cytoplasm; cytoplasm; melanosome; plasma membrane; endosome membrane; nerve terminal; endosome

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; GDP-dissociation inhibitor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; intracellular protein transport; regulation of endosome size; early endosome to late endosome transport; positive regulation of smooth muscle cell proliferation; regulation of endocytosis; endocytosis; regulation of filopodium formation; positive regulation of smooth muscle cell migration; blood coagulation; Rab protein signal transduction; positive regulation of exocytosis