Human MAT2B (Methionine adenosyltransferase 2 subunit beta) ELISA Kit | MAT2B elisa kit

Human MAT2B (Methionine adenosyltransferase 2 subunit beta) ELISA Kit

Cat. Num. AAA7606238

• Gene Names: MAT2B; TGR; MAT-II; SDR23E1; MATIIbeta; Nbla02999
• Reactivity: Human
• Synonyms: MAT2B (Methionine adenosyltransferase 2 subunit beta); Human MAT2B (Methionine adenosyltransferase 2 subunit beta) ELISA Kit; MAT2B elisa kit

• Reactivity: Human
• Sequence Length: 323

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAT2B elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MAT2B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MAT2B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MAT2B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MAT2B can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 33519455
• NCBI GeneID: 27430
• NCBI Accession #: NP_877725.1
• NCBI GenBank Nucleotide #: NM_182796.2
• Molecular Weight: 9,488 Da
• NCBI Official Full Name: methionine adenosyltransferase 2 subunit beta isoform 2
• NCBI Official Synonym Full Names: methionine adenosyltransferase 2B
• NCBI Official Symbol: MAT2B
• NCBI Official Synonym Symbols: TGR; MAT-II; SDR23E1; MATIIbeta; Nbla02999
• NCBI Protein Information: methionine adenosyltransferase 2 subunit beta
• UniProt Protein Name: Methionine adenosyltransferase 2 subunit beta
• Protein Family: Methionine adenosyltransferase
• UniProt Gene Name: MAT2B
• UniProt Synonym Gene Names: TGR; MAT II beta

NCBI Description

The protein encoded by this gene belongs to the methionine adenosyltransferase (MAT) family. MAT catalyzes the biosynthesis of S-adenosylmethionine from methionine and ATP. This protein is the regulatory beta subunit of MAT. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]

Uniprot Description

MAT2B: Non-catalytic regulatory subunit of S-adenosylmethionine synthetase 2 (MAT2A), an enzyme that catalyzes the formation of S- adenosylmethionine from methionine and ATP. Regulates the activity of S-adenosylmethionine synthetase 2 by changing its kinetic properties, rendering the enzyme more susceptible to S- adenosylmethionine inhibition. Belongs to the dTDP-4-dehydrorhamnose reductase family. MAT2B subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - cysteine and methionine; Other Amino Acids Metabolism - selenoamino acid; Transferase

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: cytosol; intracellular; methionine adenosyltransferase complex; nucleus

Molecular Function: enzyme binding; methionine adenosyltransferase regulator activity; protein binding

Biological Process: methylation; S-adenosylmethionine biosynthetic process