Western Blot (WB)
(The extracts of mouse brain (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human, Mouse Rab5a Monoclonal Antibody | anti-Rab5a antibody
Rab5a antibody
Gene Names
RAB5A; RAB5
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Synonyms
Rab5a; Monoclonal Antibody; Rab5a antibody; RAB5A member RAS oncogene family; RAB5A; member RAS oncogene family; RAB5; member RAS oncogene family RAB 5; RAB 5A; RAS associated protein RAB5A; Ras related protein Rab 5A.; anti-Rab5a antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
3A4
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
215
Applicable Applications for anti-Rab5a antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500 ~ 1,000.
Recommended starting dilution is 1:500.
Recommended starting dilution is 1:500.
Antigen Species
Human
Immunogen
Recombinant human Rab5a (1-215aa) purified from E. coli
Clone Note
Anti-human Rab5a mAb, clone 3A4, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Rab5a protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The extracts of mouse brain (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(The extracts of mouse brain (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-Rab5a antibody
Rab5a is a member of the RAS oncogene family. Rab5a is a small GTPase localized to the plasma membrane and early endosomes. It is a crucial regulatory component of the endocytic pathway. Activation of Rab5a is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex.
Product Categories/Family for anti-Rab5a antibody
References
Bucci C, et al., (1992) Cell 70 (5): 715-28; Seachrist JL, et al., (2002) J. Biol. Chem. 277 (1): 679-85
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,178 Da
NCBI Official Full Name
ras-related protein Rab-5A isoform 1
NCBI Official Synonym Full Names
RAB5A, member RAS oncogene family
NCBI Official Symbol
RAB5A
NCBI Official Synonym Symbols
RAB5
NCBI Protein Information
ras-related protein Rab-5A
UniProt Protein Name
Ras-related protein Rab-5A
Protein Family
UniProt Gene Name
RAB5A
UniProt Synonym Gene Names
RAB5
Uniprot Description
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 ().
Research Articles on Rab5a
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Product Notes
The Rab5a rab5a (Catalog #AAA200066) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Rab5a antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rab5a can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500 ~ 1,000. Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the Rab5a rab5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rab5a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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