ACBP recombinant protein

ACBP, 1-87aa, Human, His tag, E Coli

Cat. Num. AAA203475

• Gene Names: DBI; EP; ACBP; ACBD1; CCK-RP
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: ACBP; 1-87aa; Human; His tag; E Coli; Acyl-CoA-binding protein; ACBD1; CCK-RP; EP; DBI; Diazepam Binding Inhibitor Protein; ACBP recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 1-87aa
• Sequence: MGSSHHHHHH SSGLVPRGSH MSQAEFEKAA EEVRHLKTKP SDEEMLFIYG HYKQATVGDI NTERPGMLDF TGKAKWDAWN ELKGTSKEDA MKAYINKVEE LKKKYGI

• Applicable Applications for ACBP recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 2-8°C for 1 week.; For long term storage, aliquot and store at -20°C to -80°C .; Avoid repeated freezing and thawing cycles.

SDS-Page

(3 ug by SDS-PAGE under reducing conditions and visualized by coomasie blue stain.)

Related Product Information for ACBP recombinant protein

ACBP, also known as ACBD1, is a diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. Expression of ACBP is significantly induced during adipocyte differentiation. ACBP is a target for proliferator-activated receptor (PPAR)gamma, and is directly activated by PPARgamma/RXRalpha and PPARalpha/RXRalpha, but not by PPARdelta/RXRalpha. Recombinant human ACBP protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ACBP recombinant protein

Neuroscience

References

Folli F., et al. (2010) PLoS One. 5 (3):e9923. ; Manjithaya R., et al. (2010) J Cell Biol. 188 (4):537-46.;

NCBI and Uniprot Product Information

• NCBI GI #: 120433590
• NCBI GeneID: 1622
• NCBI Accession #: NP_001073331
• NCBI GenBank Nucleotide #: NM_001079862.2
• UniProt Accession #: P07108; P08869; Q4VWZ6; Q53SQ7; Q6IB48; Q9UCI8; B8ZWD2; B8ZWD6; B8ZWD7
• Molecular Weight: 12.2 kDa (107aa), confirmed by MALDI-TOF
• NCBI Official Full Name: acyl-CoA-binding protein isoform 3
• NCBI Official Synonym Full Names: diazepam binding inhibitor, acyl-CoA binding protein
• NCBI Official Symbol: DBI
• NCBI Official Synonym Symbols: EP; ACBP; ACBD1; CCK-RP
• NCBI Protein Information: acyl-CoA-binding protein
• UniProt Protein Name: Acyl-CoA-binding protein
• UniProt Gene Name: DBI
• UniProt Synonym Gene Names: ACBP; DBI; EP

NCBI Description

This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.