Western Blot (WB)
(Western Blot analysis of Human, Mouse A549 WT and KO cells, MEF, mouse brain cells showing detection of RAB1A protein using Mouse Anti-RAB1A Monoclonal Antibody, Clone 4G10 . Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3-5: KO A549 clones. Lane 6: MEF cells. Lane 7: mouse brain cells.. Load: 10ug (A549, MEF); 20ug (Mouse Brain). Primary Antibody: Mouse Anti-RAB1A Monoclonal Antibody at 1:1000.)
Mouse anti-Human, Mouse RAB1A Monoclonal Antibody | anti-RAB1A antibody
RAB1A Antibody: ATTO 488
A 1:1000 dilution was sufficient for detection of RAB1A in 10ug (A549, MEF); 20ug (Mouse Brain) lysates by ECL immunoblot analysis.
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Western Blot (WB)
(Western Blot analysis of Human, Mouse A549 WT and KO cells, MEF, mouse brain cells showing detection of RAB1A protein using Mouse Anti-RAB1A Monoclonal Antibody, Clone 4G10 . Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3-5: KO A549 clones. Lane 6: MEF cells. Lane 7: mouse brain cells.. Load: 10ug (A549, MEF); 20ug (Mouse Brain). Primary Antibody: Mouse Anti-RAB1A Monoclonal Antibody at 1:1000.)
Western Blot (WB)
(Western Blot analysis of Human, Mouse A549 WT and KO cells, MEF, mouse brain cells showing detection of RAB1A protein using Mouse Anti-RAB1A Monoclonal Antibody, Clone 4G10 . Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3-5: KO A549 clones. Lane 6: MEF cells. Lane 7: mouse brain cells.. Load: 10ug (A549, MEF); 20ug (Mouse Brain). Primary Antibody: Mouse Anti-RAB1A Monoclonal Antibody at 1:1000.)
Immunoprecipitation (IP0
(Immunoprecipitation analysis using Mouse Anti-RAB1A Monoclonal Antibody, Clone 4G10 . Tissue: HEK293 cells overexpressing RAB1A, RAB1B, and RAB1C. Species: Human. Primary Antibody: Mouse Anti-RAB1A Monoclonal Antibody .)
Immunoprecipitation (IP0
(Immunoprecipitation analysis using Mouse Anti-RAB1A Monoclonal Antibody, Clone 4G10 . Tissue: HEK293 cells overexpressing RAB1A, RAB1B, and RAB1C. Species: Human. Primary Antibody: Mouse Anti-RAB1A Monoclonal Antibody .)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Ras superfamily of GTPases. Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. This small GTPase controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms. [provided by RefSeq, Oct 2008]
Uniprot Description
RAB1A: Probably required for transit of protein from the ER through Golgi compartment. Binds GTP and GDP and possesses intrinsic GTPase activity. Belongs to the small GTPase superfamily. Rab family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: G protein, monomeric; G protein, monomeric, Rab; G protein
Chromosomal Location of Human Ortholog: 2p14
Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; early endosome; melanosome; cytosol
Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding
Biological Process: ER to Golgi vesicle-mediated transport; cell migration; endocytosis; virus assembly; melanosome transport; vesicle-mediated transport; growth hormone secretion; intracellular protein transport; defense response to bacterium; vesicle transport along microtubule; autophagy; cilium biogenesis; mitotic cell cycle; Rab protein signal transduction; Golgi organization and biogenesis; autophagic vacuole formation
Research Articles on RAB1A
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Product Notes
The RAB1A rab1a (Catalog #AAA8005924) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAB1A Antibody: ATTO 488 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RAB1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:1000 A 1:1000 dilution was sufficient for detection of RAB1A in 10ug (A549, MEF); 20ug (Mouse Brain) lysates by ECL immunoblot analysis. Researchers should empirically determine the suitability of the RAB1A rab1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB1A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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