Western Blot (WB)
(Western blot analysis of whole cell lysates probed with pyruvate kinase PKM antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc™ MP with 4 second exposure. Arrow points to pyruvate kinase PKM (molecular weight 60 kDa))
Mouse Pyruvate Kinase Pkm Monoclonal Antibody | anti-PKM2 antibody
Pyruvate Kinase Pkm Antibody with Control Lysate
Western Blotting: Detects a band of approximately 60 kDa in HeLa cell lysate.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with pyruvate kinase PKM antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc™ MP with 4 second exposure. Arrow points to pyruvate kinase PKM (molecular weight 60 kDa))
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with pyruvate kinase PKM antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc™ MP with 4 second exposure. Arrow points to pyruvate kinase PKM (molecular weight 60 kDa))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
Uniprot Description
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Research Articles on PKM2
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Product Notes
The PKM2 pkm (Catalog #AAA225495) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Pyruvate Kinase Pkm Antibody with Control Lysate reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Pyruvate Kinase Pkm can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 Western Blotting: Detects a band of approximately 60 kDa in HeLa cell lysate. Researchers should empirically determine the suitability of the PKM2 pkm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pyruvate Kinase Pkm, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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