Mouse anti-Human, Mouse PURB Monoclonal Antibody | anti-PURB antibody

PURB Antibody (C-term)

Cat. Num. AAA9232281

• Gene Names: PURB; PURBETA
• Reactivity: Human, Mouse
• Applications: Western Blot
• Synonyms: PURB; Monoclonal Antibody; PURB Antibody (C-term); Transcriptional activator protein Pur-beta; Purine-rich element-binding protein B; anti-PURB antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1,kappa
• Clone Number: 2117CT1176.48.6
• Specificity: This PURB antibody is generated from a mouse immunized with a recombinant protein of human PURB.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 312

• Applicable Applications for anti-PURB antibody: Western Blot (WB)
• Application Notes: WB: ~1:8000
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes: Anti-PURB Antibody (C-term) at 1:8000 dilutionLane 1: Jurkat whole cell lysateLane 2: HeLa whole cell lysateLane 3: NCI-H460 whole cell lysateLane 4: HepG2 whole cell lysateLane 5: MCF-7 whole cell lysateLane 6: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size: 33 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-PURB antibody

Has capacity to bind repeated elements in single- stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine- rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs (By similarity).
Purified Mouse Monoclonal Antibody (Mab)

NCBI and Uniprot Product Information

• NCBI GI #: 15147219
• NCBI GeneID: 5814
• NCBI Accession #: NP_150093
• NCBI GenBank Nucleotide #: NP_150093.1
• UniProt Accession #: Q96QR8
• Molecular Weight: 33241
• NCBI Official Full Name: transcriptional activator protein Pur-beta
• NCBI Official Synonym Full Names: purine rich element binding protein B
• NCBI Official Symbol: PURB
• NCBI Official Synonym Symbols: PURBETA
• NCBI Protein Information: transcriptional activator protein Pur-beta
• UniProt Protein Name: Transcriptional activator protein Pur-beta
• Protein Family: Transcriptional activator protein
• UniProt Gene Name: PURB

NCBI Description

This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]

Uniprot Description

Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs ().