Mouse anti-Human CD147 Monoclonal Antibody | anti-CD147 antibody

anti-human CD147-Purified Preservative Free

Cat. Num. AAA666187

• Gene Names: BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Purity: Purified Preservative Free
• Synonyms: CD147; Monoclonal Antibody; anti-human CD147-Purified Preservative Free; Monoclonal anti-human CD147 (neurothelin/basigin); anti-CD147 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: UM-8D6
• Purity/Purification: Purified Preservative Free
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 269

• Applicable Applications for anti-CD147 antibody: Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
• Performance: Five x 105 cultured human Raji cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD147 antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.81 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
• Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
• Immunogen: Human T cell line Molt 13
• Preparation and Storage: Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD147 antibody

Information: Human CD147 is a transmembrane glycoprotein expressed more intensely on thymocytes than on mature peripheral blood T cells. Antibody UM-8D6 recognizes the CD147 molecule of about 35-40 kd (2). UM-8D6 inhibits homotypic aggregation, adhesion to matrix proteins and migation through matrigel (1).

References

1.) Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 760-766. 2.) A.H. Kirsch, et al, (1997) Tissue Antigens 50:147-152.

NCBI and Uniprot Product Information

• NCBI GI #: 31076333
• NCBI GeneID: 682
• Molecular Weight: 22,764 Da
• NCBI Official Full Name: CD147
• NCBI Official Synonym Full Names: basigin (Ok blood group)
• NCBI Official Symbol: BSG
• NCBI Official Synonym Symbols: M6; OK; 5F7; TCSF; CD147; EMMPRIN
• NCBI Protein Information: basigin; CD147 antigen; OK blood group antigen; collagenase stimulatory factor; leukocyte activation antigen M6; extracellular matrix metalloproteinase inducer; tumor cell-derived collagenase stimulatory factor
• UniProt Protein Name: Basigin
• UniProt Gene Name: BSG
• UniProt Synonym Gene Names: EMMPRIN; TCSF
• UniProt Entry Name: BASI_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BSG: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Enriched on the surface of tumor cells. Up-regulated in gliomas. Its expression levels correlate with malignant potential of the tumor. Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with ATP1B2, MAG and L1CAM. Interacts with AJAP1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi membrane; focal adhesion; mitochondrion; membrane; integral to plasma membrane; plasma membrane; melanosome; sarcolemma; acrosomal membrane; lipid raft

Molecular Function: mannose binding; protein binding

Biological Process: response to mercury ion; cellular metabolic process; extracellular matrix disassembly; response to peptide hormone stimulus; extracellular matrix organization and biogenesis; cell surface receptor linked signal transduction; response to cAMP; decidualization; blood coagulation; pyruvate metabolic process; leukocyte migration; embryo implantation; odontogenesis of dentine-containing teeth

Disease: Blood Group--ok