Rabbit anti-Human PSGL-1/CD162 Monoclonal Antibody | anti-SELPLG antibody

Recombinant Anti-PSGL-1/CD162 Antibody, Rabbit Monoclonal

Cat. Num. AAA8107127

• Gene Names: SELPLG; CLA; CD162; PSGL1; PSGL-1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A
• Synonyms: PSGL-1/CD162; Monoclonal Antibody; Recombinant Anti-PSGL-1/CD162 Antibody; Rabbit Monoclonal; PSGL-1/CD162/SELPLG Antibody; Rabbit MAb; selectin P ligand; Anti-CD162 Antibody; Anti-CLA Antibody; Anti-PSGL-1 Antibody; Anti-PSGL1 Antibody; anti-SELPLG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 108
• Specificity: Human PSGL-1/CD162/SELPLG
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SELPLG antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IP: 0.2-1uL/mg of lysate
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human PSGL-1/CD162/SELPLG Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human PSGL-1/CD162/SELPLG (rh PSGL-1/CD162/SELPLG).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(SELPLG was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate1 uL anti-SELPLG rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SELPLG rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 43 kDaObserved band size: 120 kDa)

Western Blot (WB)

(Anti-SELPLG rabbit monoclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:43 kDaObserved band size:120 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-SELPLG antibody

P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.

References

1. Sako, D. et al., 1993, Cell. 75: 1179-1186.
2. Wilkins, P. P. et al., 1995, J. Biol. Chem. 270: 22677-22680.
3. Frenette, P. S. et al., 2000, J. Exp. Med. 191: 1413-1422.
4. Vandendries, E.R.et al., 2004, Thromb. Haemost. 92: 459-466..
5. Pouyani, T. et al., 1995, Cell. 83: 333-343.

NCBI and Uniprot Product Information

• NCBI GI #: 331284238
• NCBI GeneID: 6404
• NCBI Accession #: NP_001193538.1
• NCBI GenBank Nucleotide #: NM_001206609.1
• UniProt Accession #: Q14242; Q12775; Q6GTW7; Q8N7J7; A8K2Y0; B4DQC3; B7Z5C7; J3KMX6
• Molecular Weight: 43,201 Da
• NCBI Official Full Name: P-selectin glycoprotein ligand 1 isoform 1
• NCBI Official Synonym Full Names: selectin P ligand
• NCBI Official Symbol: SELPLG
• NCBI Official Synonym Symbols: CLA; CD162; PSGL1; PSGL-1
• NCBI Protein Information: P-selectin glycoprotein ligand 1; cutaneous lymphocyte-associated associated antigen
• UniProt Protein Name: P-selectin glycoprotein ligand 1
• Protein Family: P-selectin glycoprotein ligand
• UniProt Gene Name: SELPLG
• UniProt Synonym Gene Names: PSGL-1
• UniProt Entry Name: SELPL_HUMAN

NCBI Description

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

Uniprot Description

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; receptor binding

Biological Process: blood coagulation; cell adhesion; leukocyte tethering or rolling; leukocyte adhesive activation; leukocyte migration