Rabbit anti-Human SNX3 Polyclonal Antibody | anti-SNX3 antibody

SNX3, ID (SNX3, Sorting nexin-3, Protein SDP3) (Azide free) (HRP)

Cat. Num. AAA6349643

• Gene Names: SNX3; SDP3; Grd19; MCOPS8
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SNX3; Polyclonal Antibody; ID (SNX3; Sorting nexin-3; Protein SDP3) (Azide free) (HRP); Protein SDP3; anti-SNX3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 130

• Applicable Applications for anti-SNX3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SNX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the Central region of human SNX3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SNX3 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the SNX3 antibody detected the SNX3 protein (arrow).)

Related Product Information for anti-SNX3 antibody

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like most family members. This protein interacts with phosphatidylinositol-3-phosphate, and is involved in protein trafficking.

Product Categories/Family for anti-SNX3 antibody

Antibodies; SNX (Sorting Nexin) Proteins

References

Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Pons, V., et al. PLoS Biol. 6 (9), E214 (2008): Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008). Dong, H., et al. Acta Biochim. Biophys. Sin. (Shanghai) 39(7):540-546(2007). Kumar, R.A., et al. BMC Med. Genet. 8, 48 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 23111041
• NCBI GeneID: 8724
• NCBI Accession #: NP_690040.1
• NCBI GenBank Nucleotide #: NP_690040.1
• UniProt Accession #: O60493
• NCBI Official Full Name: sorting nexin-3 isoform b
• NCBI Official Synonym Full Names: sorting nexin 3
• NCBI Official Symbol: SNX3
• NCBI Official Synonym Symbols: SDP3; Grd19; MCOPS8
• NCBI Protein Information: sorting nexin-3
• UniProt Protein Name: Sorting nexin-3
• Protein Family: Sorting nexin
• UniProt Gene Name: SNX3
• UniProt Entry Name: SNX3_HUMAN

NCBI Description

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like most family members. This protein interacts with phosphatidylinositol-3-phosphate, and is involved in protein trafficking. A pseudogene of this gene is present on the sex chromosomes. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]

Uniprot Description

SNX3: Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol 3-phosphate (PtdIns(P3)). Plays a role in protein transport between cellular compartments. Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G. Not involved in EGFR degradation. A chromosomal aberration involving SNX3 may be a cause of microphthalmia syndromic type 8 (MCOPS8). Translocation t(6;13)(q21;q12). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS8 is a very rare congenital syndrome characterized by microcephaly, microphthalmia, ectrodactyly of the lower limbs and prognathism. Intellectual deficit has been reported. Belongs to the sorting nexin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: extrinsic to membrane; intracellular membrane-bound organelle; clathrin-coated vesicle; retromer complex; early endosome membrane; cytoplasm; early endosome; endosome membrane; cytosol

Molecular Function: protein binding; phosphatidylinositol-5-phosphate binding; protein phosphatase binding; phosphatidylinositol 3-phosphate binding

Biological Process: hemoglobin biosynthetic process; negative regulation of phagocytosis; regulation of cellular protein metabolic process; regulation of intracellular protein transport; response to bacterium; negative regulation of virion penetration into host cell; vesicle organization and biogenesis; regulation of Wnt receptor signaling pathway; membrane invagination; transferrin transport; protein to membrane docking; negative regulation of protein transport; negative regulation of protein catabolic process

Disease: Microphthalmia, Syndromic 8