Mouse PSAT1 Monoclonal Antibody | anti-PSAT1 antibody

PSAT1 (phosphoserine Aminotransferase 1, EPIP, MGC1460, PSA, PSAT) (HRP)

Cat. Num. AAA6180812

• Gene Names: PSAT1; PSA; EPIP; NLS2; PSAT; PSATD
• Applications: ELISA
• Purity: Purified
• Synonyms: PSAT1; Monoclonal Antibody; PSAT1 (phosphoserine Aminotransferase 1; EPIP; MGC1460; PSA; PSAT) (HRP); phosphoserine Aminotransferase 1; PSAT; anti-PSAT1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 1C2
• Specificity: Recognizes PSAT1.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-PSAT1 antibody: ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: PSAT1 (NP_478059, 262aa-370aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged PSAT1 is 0.03 ng/ml as a capture antibody.)

Product Categories/Family for anti-PSAT1 antibody

Antibodies; Enzymes; Aminotransferase

NCBI and Uniprot Product Information

• NCBI GI #: 17402893
• NCBI GeneID: 29968
• NCBI Accession #: NP_478059
• NCBI GenBank Nucleotide #: NM_058179.3
• UniProt Accession #: Q9Y617; Q5T7G5; Q5T7G6; Q96AW2; Q9BQ12
• Molecular Weight: 42.9 kDa (394aa), confirmed by MALDI-TOF
• NCBI Official Full Name: phosphoserine aminotransferase isoform 1
• NCBI Official Synonym Full Names: phosphoserine aminotransferase 1
• NCBI Official Symbol: PSAT1
• NCBI Official Synonym Symbols: PSA; EPIP; NLS2; PSAT; PSATD
• NCBI Protein Information: phosphoserine aminotransferase
• UniProt Protein Name: Phosphoserine aminotransferase
• Protein Family: Phosphoserine aminotransferase
• UniProt Gene Name: PSAT1
• UniProt Synonym Gene Names: PSA; PSAT
• UniProt Entry Name: SERC_HUMAN

NCBI Description

This gene encodes a member of the class-V pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is a phosphoserine aminotransferase and decreased expression may be associated with schizophrenia. Mutations in this gene are also associated with phosphoserine aminotransferase deficiency. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, and 8. [provided by RefSeq, Jul 2013]

Uniprot Description

PSAT1: Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine. Defects in PSAT1 are the cause of phosphoserine aminotransferase deficiency (PSATD). PSATD is characterized biochemically by low plasma and cerebrospinal fluid concentrations of serine and glycine and clinically by intractable seizures, acquired microcephaly, hypertonia, and psychomotor retardation. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.6.1.52; Amino Acid Metabolism - glycine, serine and threonine; Cofactor and Vitamin Metabolism - vitamin B6

Chromosomal Location of Human Ortholog: 9q21.2

Cellular Component: cytoplasm; cytosol

Molecular Function: phosphoserine transaminase activity; pyridoxal phosphate binding

Biological Process: L-serine biosynthetic process; pyridoxine biosynthetic process; amino acid biosynthetic process

Disease: Phosphoserine Aminotransferase Deficiency; Neu-laxova Syndrome 2