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Mouse anti-Human pS2 Monoclonal Antibody | anti-pS2 antibody

pS2 (BCEI, Breast Cancer Estrogen Inducible Protein, Gastrointestinal Trefoil Protein, Gastrointestinal trefoil Protein pS2, HP1A, HPS2, pNR2, pNR-2, TFF1, Trefoil Factor 1) (APC)

Gene Names
TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
pS2; Monoclonal Antibody; pS2 (BCEI; Breast Cancer Estrogen Inducible Protein; Gastrointestinal Trefoil Protein; Gastrointestinal trefoil Protein pS2; HP1A; HPS2; pNR2; pNR-2; TFF1; Trefoil Factor 1) (APC); BCEI; Trefoil Factor 1; anti-pS2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
SPM573
Specificity
Recognizes pS2. Species Crossreactivity: Human and Cynomolgus Monkey. Others-not known
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-pS2 antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IP: 0.5-1ug/500ug protein lysate
FC/FACS: 0.5-1ug/million cells
IHC: 0.5-1.0ug/ml for 30 minutes at RT
IF: 0.5-1ug/ml
WB: 0.5-1.0ug/ml
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide of 28 amino acid residues corresponding to CFDDTVRGVPWCFYPNTIDVPPEEECEF (aa57-84) from the C-terminus of human pS2. Cellular Localization: Cytoplasmic
Conjugate
APC
Positive Control
Spent medium of MCF-7 cells, treated with estrogen. Normal stomach. About 60% of breast carcinomas are positive for pS2, especially those that are also positive for estrogen and/or progesterone receptor.
Special Handling
Light sensitive
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.
Product Categories/Family for anti-pS2 antibody
References
1. Williams R, et. al. Human Pathology, 1996, 27(12):1259-66.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
9,150 Da
NCBI Official Full Name
trefoil factor 1
NCBI Official Synonym Full Names
trefoil factor 1
NCBI Official Symbol
TFF1
NCBI Official Synonym Symbols
pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
NCBI Protein Information
trefoil factor 1; protein pS2; polypeptide P1.A; gastrointestinal trefoil protein pS2; breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence
UniProt Protein Name
Trefoil factor 1
Protein Family
UniProt Gene Name
TFF1
UniProt Synonym Gene Names
BCEI; PS2; hP1.A
UniProt Entry Name
TFF1_HUMAN

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

TFF1: Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular space

Molecular Function: protein binding; growth factor activity

Biological Process: negative regulation of cell proliferation; response to peptide hormone stimulus; maintenance of gastrointestinal epithelium; digestion; carbohydrate metabolic process; cell differentiation; response to iron ion; response to estradiol stimulus

Research Articles on pS2

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Product Notes

The pS2 tff1 (Catalog #AAA6124481) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The pS2 (BCEI, Breast Cancer Estrogen Inducible Protein, Gastrointestinal Trefoil Protein, Gastrointestinal trefoil Protein pS2, HP1A, HPS2, pNR2, pNR-2, TFF1, Trefoil Factor 1) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's pS2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). IP: 0.5-1ug/500ug protein lysate FC/FACS: 0.5-1ug/million cells IHC: 0.5-1.0ug/ml for 30 minutes at RT IF: 0.5-1ug/ml WB: 0.5-1.0ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the pS2 tff1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "pS2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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