Mouse major histocompatibility complex-III (MHC III / HLA-III) ELISA Kit | MHC III / HLA-III elisa kit

Mouse major histocompatibility complex-III (MHC III / HLA-III) ELISA Kit

Cat. Num. AAA264562

• Gene Names: CIITA; C2TA; NLRA; MHC2TA; CIITAIV
• Reactivity: Mouse
• Synonyms: major histocompatibility complex-III (MHC III / HLA-III); Mouse major histocompatibility complex-III (MHC III / HLA-III) ELISA Kit; MHC III / HLA-III elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 546

• Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MHCIII/HLA-III concentration
• Assay Type: Sandwich
• Detection Range: 100 ng/ml-1.56 ng/ml
• Sensitivity: 0.5 ng/ml.
• Intra-assay Precision: ? 8%
• Inter-assay Precision: ? 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MHC III / HLA-III elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse MHCIII/HLA-III monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 556695497
• NCBI GeneID: 4261
• NCBI Accession #: NP_001273332.1
• NCBI GenBank Nucleotide #: NM_001286403.1
• Molecular Weight: 101,661 Da
• NCBI Official Full Name: MHC class II transactivator isoform 3
• NCBI Official Synonym Full Names: class II, major histocompatibility complex, transactivator
• NCBI Official Symbol: CIITA
• NCBI Official Synonym Symbols: C2TA; NLRA; MHC2TA; CIITAIV
• NCBI Protein Information: MHC class II transactivator; NLR family, acid domain containing; MHC class II transactivator type III; nucleotide-binding oligomerization domain, leucine rich repeat and acid domain containing
• UniProt Protein Name: MHC class II transactivator
• UniProt Gene Name: CIITA
• UniProt Synonym Gene Names: MHC2TA; CIITA
• UniProt Entry Name: C2TA_HUMAN

NCBI Description

This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. The protein also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Uniprot Description

CIITA: a non-DNA binding transactivator that functions both in constitutive and inducible MHC Class II expression. Defects are a cause of bare lymphocyte syndrome type II (BLS II), a form of severe combined immunodeficiency disease (SCID) characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, an absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production.

Protein type: DNA-binding; Transcription, coactivator/corepressor; EC 2.7.11.1

Chromosomal Location of Human Ortholog: 16p13

Cellular Component: nucleoplasm; PML body

Molecular Function: protein C-terminus binding; protein binding; GTP binding; DNA binding; transcription activator binding; transcription coactivator activity; transferase activity, transferring acyl groups; protein complex binding; kinase activity; ATP binding

Biological Process: response to antibiotic; transcription, DNA-dependent; negative regulation of collagen biosynthetic process; cytokine and chemokine mediated signaling pathway; positive regulation of MHC class II biosynthetic process; positive regulation of transcription, DNA-dependent; positive regulation of MHC class I biosynthetic process; immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; phosphorylation

Disease: Rheumatoid Arthritis; Bare Lymphocyte Syndrome, Type Ii