Mouse Protocadherin FAT2 Monoclonal Antibody | anti-FAT2 antibody

Protocadherin FAT2 (FAT Atypical Cadherin 2)

Cat. Num. AAA439953

• Gene Names: FAT2; CDHF8; CDHR9; HFAT2; MEGF1; SCA45
• Reactivity: Human. Others not known.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: Protocadherin FAT2; Monoclonal Antibody; Protocadherin FAT2 (FAT Atypical Cadherin 2); FAT tumor suppressor homolog 2 (HFAT2); cadherin family member 8 (CDHF8); cadherin-related family member 9 (CDHR9); multiple EGF-like domains protein 1; multiple epidermal growth factor-like domains 1 (MEGF1); multiple epidermal growth factor-like domains protein 1; protocadherin FAT2.; anti-FAT2 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 8C5
• Specificity: Recognizes a protein of 480kDa, which is identified as FAT2. The cadherins represent a family of Ca2+-dependent adhesion molecules that function to mediate cell-to-cell binding that is critical for the maintenance of structure and morphogenesis. Cadherins each contain a large extracellular domain at the N-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short C-terminal intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. The cadherin superfamily includes cadherins, protocadherins, desmogleins and desmocollins. FAT2 (FAT tumor suppressor homolog 2) is a single-pass type I membrane protein that belongs to the protocadherin subfamily of cadherins. FAT2 contains one Laminin G-like domain, two EGF-like domains and 32 cadherin domains and is believed to function as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 4349

• Applicable Applications for anti-FAT2 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: FC/FACS: 0.5-1ug/million cells; IF: 1-2ug/ml; IHC-F: 0.5-1ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Immunogen: Purified recombinant human FAT2 fusion protein
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 5q33.1
• Positive Control: Esophagus, Cervix, Uterus, Tonsil or Cerebellum.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded Human Cervical Carcinoma stained with Protocadherin FAT2 Monoclonal Antibody (8C5).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Protocadherin FAT2 Monoclonal Antibody (8C5).)

References

Sano, K., et al. 1993. Protocadherins: a large family of cadherin-related molecules in central nervous system. EMBO J. 12: 2249-2256. Suzuki, S.T. 2000. Recent progress in protocadherin research. Exp. Cell Res. 261: 13-18.

NCBI and Uniprot Product Information

• NCBI GI #: 13787217
• NCBI GeneID: 2196
• NCBI Accession #: NP_001438.1
• NCBI GenBank Nucleotide #: NM_001447.2
• UniProt Accession #: Q9NYQ8; O75091; Q9NSR7
• Molecular Weight: 480kDa
• NCBI Official Full Name: protocadherin Fat 2
• NCBI Official Synonym Full Names: FAT atypical cadherin 2
• NCBI Official Symbol: FAT2
• NCBI Official Synonym Symbols: CDHF8; CDHR9; HFAT2; MEGF1; SCA45
• NCBI Protein Information: protocadherin Fat 2
• UniProt Protein Name: Protocadherin Fat 2
• Protein Family: Protocadherin
• UniProt Gene Name: FAT2
• UniProt Synonym Gene Names: CDHF8; KIAA0811; MEGF1; hFat2; Multiple EGF-like domains protein 1

NCBI Description

This gene is the second identified human homolog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. In addition to containing 34 tandem cadherin-type repeats, the gene product has two epidermal growth factor (EGF)-like repeats and one laminin G domain. This protein most likely functions as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays a role in the migration of epidermal cells. May modulate the extracellular space surronding parallel fibers of cerebellar during development ().