Mouse Desmocollin-2/3 Monoclonal Antibody | anti-DSC2 antibody

Desmocollin-2/3

Cat. Num. AAA439911

• Gene Names: DSC2; DG2; DSC3; CDHF2; ARVD11; DGII/III
• Reactivity: Human, Mouse, Rat. Others not known.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: Desmocollin-2/3; Monoclonal Antibody; ARVD11; Cadherin family member 2; CDHF2; Desmocollin-2; Desmocollin-3; Desmosomal glycoprotein II and III; Desmosomal glycoprotein II; Desmosomal glycoprotein III; DG2; DGII/III; DSC2; DSC3; anti-DSC2 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 7G6
• Specificity: Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of desmocollin-2 varies in the basal and suprabasal layers. And desmocollin-3 is expressed more evenly throughout the suprabasal layers.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 847

• Applicable Applications for anti-DSC2 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: FC/FACS: 0.5-1ug/million cells; IF: 1-2ug/ml; WB: 0.5-1.0ug/ml; IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Immunogen: Human desmocollin-2 extracellular domain
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 18q12.1
• Positive Control: HeLa cells. Skin
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Skin stained with Desmocollin-2/3 Monoclonal Antibody (7G6).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Skin stained with Desmocollin-2/3 Mouse Monoclonal Antibody (7G6).)

SDS_PAGE (SDS)

(SDS-PAGE Analysis Purified Desmocollin-2/3 Mouse Monoclonal Antibody (7G6). Confirmation of Purity and Integrity of Antibody.)

References

Buxton, RS; Cowin, P; Franke, WW; Garrod, DR; Green, KJ; King, IA; Koch, PJ; Magee, AI; Rees, DA; Stanley, JR; Steinberg, MS. Nomenclature of the desmosomal cadherins. J Cell Biol 1993, 121:481-483.

NCBI and Uniprot Product Information

• NCBI GI #: 13435366
• NCBI GeneID: 1824
• NCBI Accession #: NP_004940.1
• NCBI GenBank Nucleotide #: NM_004949.4
• UniProt Accession #: Q02487; P01233
• Molecular Weight: 110kDa (DSC2); 100kDa (DSC3)
• NCBI Official Full Name: desmocollin-2 isoform Dsc2b preproprotein
• NCBI Official Synonym Full Names: desmocollin 2
• NCBI Official Symbol: DSC2
• NCBI Official Synonym Symbols: DG2; DSC3; CDHF2; ARVD11; DGII/III
• NCBI Protein Information: desmocollin-2
• UniProt Protein Name: Desmocollin-2
• Protein Family: Desmocollin
• UniProt Gene Name: DSC2

NCBI Description

This gene encodes a member of the desmocollin protein subfamily. Desmocollins, along with desmogleins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmocollin family members on chromosome 18. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11, and reduced protein expression has been described in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]

Uniprot Description

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.