Human Fatty Acid Desaturase 3 (FADS3) ELISA Kit | FADS3 elisa kit

Human Fatty Acid Desaturase 3 (FADS3) ELISA Kit

Cat. Num. AAA2023795

• Gene Names: FADS3; CYB5RP; LLCDL3
• Reactivity: Human
• Synonyms: Fatty Acid Desaturase 3 (FADS3); Human Fatty Acid Desaturase 3 (FADS3) ELISA Kit; CYB5RP; Delta-9-Desaturase; Cytochrome b5-related protein; FADS3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fatty Acid Desaturase 3 (FADS3).; No significant cross-reactivity or interference between Fatty Acid Desaturase 3 (FADS3) and analogues was observed.
• Sequence Length: 445

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.118ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Desaturase 3 (FADS3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Desaturase 3 (FADS3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FADS3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Desaturase 3 (FADS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Desaturase 3 (FADS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Desaturase 3 (FADS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Desaturase 3 (FADS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FADS3 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 13375616
• NCBI GeneID: 3995
• NCBI Accession #: NP_068373.1
• NCBI GenBank Nucleotide #: NM_021727.4
• UniProt Accession #: Q9Y5Q0; O60426
• Molecular Weight: 51,145 Da
• NCBI Official Full Name: fatty acid desaturase 3
• NCBI Official Synonym Full Names: fatty acid desaturase 3
• NCBI Official Symbol: FADS3
• NCBI Official Synonym Symbols: CYB5RP; LLCDL3
• NCBI Protein Information: fatty acid desaturase 3
• UniProt Protein Name: Fatty acid desaturase 3
• Protein Family: Fatty acid desaturase
• UniProt Gene Name: FADS3
• UniProt Synonym Gene Names: CYB5RP
• UniProt Entry Name: FADS3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]

Uniprot Description

FADS3: is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, integral; Oxidoreductase; EC 1.14.19.-; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q12-q13.1

Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane

Molecular Function: iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water

Biological Process: unsaturated fatty acid biosynthetic process